Detection and Metabolism of Phenolic Compounds by Agrobacterium tumefaciens
根癌农杆菌对酚类化合物的检测和代谢
基本信息
- 批准号:9904917
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing grant
- 财政年份:1999
- 资助国家:美国
- 起止时间:1999-09-01 至 2002-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Winans, Stephen C.MCB-9904917Abstract Bacteria that live in association with eukaryotic hosts often have intricate mechanisms to detect host-released chemical signals. This theme is well-studied in the plant pathogen Agrobacterium tumefaciens, which detects several classes of plant-released signals. This project focuses on the perception of a class of phenolic compounds that are released from plant wound sites. These compounds are detected by a two-component system consisting of the transmembrane histidine kinase VirA and the cytoplasmic response regulator VirG, which direct the induction of the vir regulon. Products of the vir regulon mediate the transfer of oncogenic DNA fragments from the bacterium to the nuclei of host plant cells, resulting in crown gall tumors. VirA is a dimeric protein that has four domains: the kinase itself and three domains that modulate kinase activity. In the past, functions have been assigned to each domain by systematically ablating each one at the genetic level. In this project, genetic techniques will be used to create VirA heterodimers containing just one phosphodonor andone phosphorecipient and the role of each domain will be determined in vivo. Soluble VirA fragments will be tested for binding of phenolic inducers and phenolic-responsive autophosphorylation and phosphotransfer reactions. The Winans' laboratory recently determined that one of the operons in the vir regulon (virH) directs the destruction of phenolic vir-inducers. For example, wild type strains can demethylate ferulic acid (a strong vir gene inducer), creating caffeic acid (a noninducer), while virH2 mutants fail to carry out this conversion. Whether related phenolic compounds are substrates for VirH2 or for the closely related protein VirH1 will be addressed. They will also test whether the products of these reactions are further catabolized by other A. tumefaciens proteins. Finally, they will learn what biological role these enzymes play in plant colonization. All pathogens must detect their hosts and make appropriate responses. Detection is generally carried out using olfactory mechanisms (essentially, by sense of smell). This project will provide use genetic and biochemical techniques to learn how a particular plant pathogen (Agrobacterium tumefaciens) detects host plants. It was recently discovered that Agrobacterium can destroy these signal molecules, and this project willstudy whether all types of these molecules are destroyed, or only certain ones. It will also study the overall effects of these reactions on the ability of this bacterium to colonize its host. These studies will provide fundamental information about host-pathogen interactions that will be useful in combating this and other pathogens.
Winans,Stephen C.MCB-9904917摘要与真核宿主一起生活的细菌通常具有复杂的机制来检测宿主释放的化学信号。这一主题在植物病原体根癌农杆菌中得到了很好的研究,根癌农杆菌检测几类植物释放的信号。该项目的重点是感知一类酚类化合物,从植物伤口部位释放。这些化合物通过由跨膜组氨酸激酶VirA和细胞质反应调节剂VirG组成的双组分系统检测,所述系统指导vir调节子的诱导。vir调节子的产物介导致癌DNA片段从细菌转移到宿主植物细胞的细胞核,导致冠瘿瘤。VirA是一种二聚体蛋白,具有四个结构域:激酶本身和调节激酶活性的三个结构域。在过去,通过在基因水平上系统地切除每个功能域来分配每个功能域。在这个项目中,遗传技术将被用来创建病毒A异源二聚体只含有一个磷酸供体和一个磷酸受体和每个域的作用将在体内确定。将检测可溶性VirA片段与酚类诱导剂的结合以及酚类响应性自磷酸化和磷酸转移反应。Winans的实验室最近确定,vir调节子(virH)中的一个操纵子指导酚类病毒诱导剂的破坏。例如,野生型菌株可以使阿魏酸(一种强vir基因诱导剂)脱甲基,产生咖啡酸(一种非诱导剂),而virH 2突变体不能进行这种转化。无论是相关的酚类化合物的底物VirH 2或密切相关的蛋白质VirH 1将得到解决。他们还将测试这些反应的产物是否被其他A.根瘤菌蛋白质。最后,他们将了解这些酶在植物定殖中发挥的生物学作用。 所有病原体都必须检测到它们的宿主并作出适当的反应。检测通常使用嗅觉机制(基本上是通过嗅觉)进行。本计画将利用遗传与生化技术,研究特定植物病原体(农杆菌)如何侦测寄主植物。最近发现,农杆菌可以破坏这些信号分子,本项目将研究是否所有类型的这些分子都被破坏,或仅某些分子被破坏。它还将研究这些反应对这种细菌在其宿主中定植能力的总体影响。这些研究将提供关于宿主-病原体相互作用的基本信息,这将有助于对抗这种和其他病原体。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Stephen Winans其他文献
Stephen Winans的其他文献
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{{ truncateString('Stephen Winans', 18)}}的其他基金
Conference: 24th Annual Crown Gall Conference, to be held at Cornell University, Noveember 22-23, 2003
会议:第 24 届皇冠加仑年度会议,将于 2003 年 11 月 22-23 日在康奈尔大学举行
- 批准号:
0407100 - 财政年份:2004
- 资助金额:
-- - 项目类别:
Standard Grant
Conference: Microbial Cell-Cell Signalling, to be held in Snowbird, Utah, July 6-9, 2001.
会议:微生物细胞间信号转导,将于 2001 年 7 月 6-9 日在犹他州斯诺伯德举行。
- 批准号:
0113022 - 财政年份:2001
- 资助金额:
-- - 项目类别:
Standard Grant
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