Competition of transcription factors in abiotic signalling

非生物信号传导中转录因子的竞争

• 批准号: 133754038
• 负责人: Professorin Dr. Margarete Baier
• 金额: --
• 依托单位: Arbeitsgruppe Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/133754038?language=en
• 关键词: Competition; transcription; factors; abiotic; signalling

ERF1b-proteins are a family of eight AP2-type transcription factors in Arabidopsis thaliana. The DNA-binding domains are highly conserved, while the C- and N-termini are variable. The high similarity of the DNA-binding domains, the potential for complementation of DREBs and CBFs and the similarity of the experimentally verified DNA-binding sites with those of salinity-, drought- and ABA-regulated transcription factors indicate an integrative, complementary or competitive function of ERF1b/Rap2.4 proteins in abiotic stress signalling networks. In the proposed project, the Arabidopsis ERF1b/Rap2.4 proteins will be analysed in vitro for their redox sensitivity and their specificity to drought-, ABA and stress regulated DNA-binding motifs. In parallel, they will be characterized in planta for their physiological function and competition strength by comparison of T-DNA insertion lines, overexpressor lines and heterologous complementation lines.

ERF1b蛋白是拟南芥中八个AP2型转录因子家族的成员。DNA结合区高度保守，而C-末端和N-末端是可变的。DNA结合域的高度相似性，DREB和CBF的互补潜力，以及实验验证的DNA结合位点与盐、干旱和ABA调节的转录因子的相似性，表明ERF1b/Rap2.4蛋白在非生物胁迫信号网络中具有整合、互补或竞争的功能。在拟议的项目中，将在体外分析拟南芥ERF1b/Rap2.4蛋白对氧化还原的敏感性以及对干旱、ABA和胁迫调节的DNA结合基序的特异性。同时，通过T-DNA插入系、超表达系和异源互补系的比较，对它们在植物中的生理功能和竞争能力进行比较。