The tAPX-NDH interplay: Its control and signalling impact

tAPX-NDH 相互作用：其控制和信号传导影响

• 批准号: 513870082
• 负责人: Professorin Dr. Margarete Baier
• 金额: --
• 依托单位: Arbeitsgruppe Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/513870082?language=en
• 关键词: tAPX; NDH; interplay; Its; control

Expression of thylakoid-bound ascorbate peroxidase (tAPX) slowly increases for several days in the poststress phase, which leads over time to accumulation of the transcripts and the enzyme to higher levels than the pre-stress ones. Our recent work demonstrated that post-stress tAPX regulation is part of the plant stress memory and of causal importance for specification of future stress signalling. We propose that tAPX dynamically controls initiation of chloroplast-to-nucleus signalling in the thylakoid membrane by competing with plastid NDH (ferredoxin-dependent plastoquinone reductase) for interaction with the donor site of photosystem I.In our future project, we will analyse the control and signalling impact of the tAPX-NDH interplay. We will investigate the distribution of tAPX, of the photosystems, light harvesting complexes and NDH in the thylakoid membrane with respect to tAPX abundance and diurnal tAPX regulation and analyse the micro-environment of tAPX mass-spectrometrically. To investigate the driving forces regulating the tAPX-NDH interplay, we will study tAPX and NDH regulation in a series of differentiating genetic backgrounds. We will extent a recent RNA-Seq study on tAPX-specific gene regulation to differentiate the NDH activity and NDH structural components in tAPX-dependent gene regulation.

类囊体结合的抗坏血酸过氧化物酶（tAPX）的表达缓慢增加了几天，在应激后阶段，这导致随着时间的推移，积累的成绩单和酶的水平高于预应力。我们最近的工作表明，胁迫后tAPX调节是植物胁迫记忆的一部分，对未来胁迫信号的规范具有因果重要性。我们建议，tAPX动态控制启动叶绿体到细胞核的信号在类囊体膜的竞争与质体NDH（铁氧还蛋白依赖性质体醌还原酶）的相互作用与光系统I的供体位点，在我们未来的项目中，我们将分析的tAPX-NDH相互作用的控制和信号的影响。我们将研究tAPX的分布，光系统，捕光复合物和NDH在类囊体膜上的tAPX丰度和昼夜tAPX调节，并分析tAPX的微环境质谱。为了研究调控tAPX-NDH相互作用的驱动力，我们将在一系列不同的遗传背景下研究tAPX和NDH的调控。我们将扩大最近的RNA-Seq研究tAPX特异性基因调控，以区分NDH活性和NDH结构组件在tAPX依赖性基因调控。