U.S.-France Cooperative Research: Changing a Plant Beta-Oxygen-Glucosidase to a Beta-Sulfur-Glucosidase or Vice Versa

美法合作研究：将植物 β-氧-葡萄糖苷酶改为 β-硫-葡萄糖苷酶，反之亦然

• 批准号: 9910088
• 负责人: Asim Esen
• 金额: $ 1.75万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-15 至 2006-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9910088&HistoricalAwards=false
• 关键词: U.S.; France; Cooperative; Research; Changing

9910088EsenThis three-year award for U.S.-France cooperative research in plant biochemistry involves Asim Esen and David R. Bevan of Virginia Polytechnic Institute and State University and Bernard Henrissat and Mirjam Czjzek of the Macromolecular Biology Laboratory in Marseilles, France. They will collaborate on the crystal structure and the structure-function relationships of beta-glucosidases a widespread plant enzyme. The investigators have chosen maize beta glucosidase and white mustard myrosinase as model systems because these enzymes exhibit subtle substrate and bond specificity. Using domain-swapping, site-directed mutagenesis and x-ray crystallography in combination with 3-D structure determination and modeling, the investigators propose to understand the mechanism of the enzymes' substrate and bond specificity.The project takes advantage of French expertise in crystallography and will advance fundamental understanding of the biochemical and physiological processes in which beta glucosidases are involved. Because these enzymes are found in many plants, basic knowledge about their structure and function may have practical importance to enzyme engineering in general and to applied research on biomass conversion, plant productivity and defense. This award will support the incremental costs of this international collaboration; that is, the travel to Marseilles, France and subsistence expenses of the U.S. investigators, postdoctoral researcher and graduate students.

9910088 Esen美国-法国植物生物化学合作研究涉及Asim Esen和大卫R.弗吉尼亚理工学院和州立大学的Bevan以及法国马赛大分子生物学实验室的Bernard Henrissat和Mirjam Czjzek。 他们将在晶体结构和β-葡萄糖苷酶（一种广泛存在的植物酶）的结构-功能关系方面进行合作。 研究者选择玉米β-葡萄糖苷酶和白色芥子酶作为模型系统，因为这些酶表现出微妙的底物和键特异性。 利用结构域交换、定点突变和X射线晶体学，结合三维结构测定和建模，研究人员建议了解酶的底物和键特异性的机制。该项目利用法国在晶体学方面的专业知识，将促进对β葡萄糖苷酶参与的生化和生理过程的基本理解。 由于这些酶存在于许多植物中，关于它们的结构和功能的基本知识对于酶工程以及生物质转化、植物生产力和防御的应用研究具有实际重要性。 该奖项将支持这项国际合作的增量成本，即前往法国马赛的旅费和美国调查人员、博士后研究人员和研究生的生活费用。