SBIR Phase I: Cottonseed Trait Development Using Chimeraplasty

这个小企业创新研究第一阶段项目将研究嵌合成形术在植物中的应用。嵌合成形术是目前在原核和真核生物系统中探索的一种新技术，它利用由DNA和修饰RNA组成的嵌合、自互补的寡核苷酸在体内靶向和突变基因。该项目的第一阶段将使用拟南芥模型系统来评估这项新技术在修改复杂生物合成途径中的适用性。后续第二阶段的资金将用于将第一阶段学到的策略应用于商业油籽作物。植物脂质合成在许多油料作物中具有许多潜在的性状发育机会。第一阶段项目的主要目标是用这种令人兴奋的体内基因组修饰技术来修饰拟南拟南植物中一个特征良好的基因。这一目标的完成将导致该技术转化为特定的特性和产品开发计划。该项目完成后，将生产出一种独特而稳定的非转基因改性油产品，这是市场上从未见过的。一种新型棉籽油产品的研制。该产品将是一种稳定的非转基因基因组修饰，因此具有快速进入市场的潜力。该产品还将具有植物油市场上从未见过的全新油籽特性。

This Small Business Innovation Research Phase 1 project will study the use of chimeraplasty in plants. Chimeraplasty is the use of a novel technology currently being explored in prokaryotic and eukaryotic systems which utilizes chimeric, self-complimentary oligonucleotides comprised of DNA and modified RNA to target and mutate genes in vivo. Phase 1 of this project will use the model system of Arabidopsis thaliana to assess the applicability of this new technology to modify a complex biosynthetic pathway. Follow-onPhase 2 funding will be used to apply the strategies learned in Phase 1 to a commercial oilseed crop. Lipid synthesis in plants has many potential trait development opportunities in many oilseed crops. The major objective of this phase 1 project is to modify a well-characterized gene in A. thaliana with this new exciting in vivo genome modification technology. Completion of this objective will lead to the translation of this technology into a specific trait and product development program. When complete this program will have produced a unique and stable non-transgenic modified-oil product, never before seen in the market place. The development of a novel cottonseed oil product. This product will be a stable genome modification that is non-transgenic and therefore has the potential to quickly reach the market. The product will also have completely new oilseed characteristics never before seen in the vegetable oils market.