Automated Sequencing at Colgate University

奖项摘要项目标题：高露洁大学自动测序NSF提案编号：9970204PI：Damhnait McHughCo-Pi：Robert S.CoyneCo-Pi：Barbara C.HoopesCo-Pi：James H.Leebens-MackCo-Pi：Heather B.McDonald该奖项与高露洁大学的资金相结合，将为高露洁大学提供一个中央现场DNA测序和基因分型设施。该设施将由一台ABI310棱镜遗传分析仪(ABI 310)和包括热循环仪、微型离心机和计算机工作站在内的辅助设备组成。该设施将使高露洁大学的教职员工、研究人员和学生能够轻松高效地为细胞和分子生物学以及进化、系统学和保护生物学的研究项目进行DNA测序和基因分型。具体地说，该设施将用于为高露洁大学教职员工的六个主要用户正在开展的各种研究项目生成数据：环节动物的系统发育分析和幼虫发育模式的进化、丝兰和丝兰蛾的共同进化、纤毛虫原生动物的大规模基因组重组、纺锤体极复制、酵母转录表达的调控以及碳酸酐酶在植物中的作用。此外，这些设备将供本科生与教职员工合作和自己的研究项目使用，附近汉密尔顿学院的未成年人用户也将可以使用该设施。

Award AbstractTitle of Project: Automated Sequencing at Colgate UniversityNSF Proposal Number: 9970204PI: Damhnait McHughCo-PI: Robert S. CoyneCo-PI: Barbara C. HoopesCo-PI: James H. Leebens-MackCo-PI: Heather B. McDonaldThis award, combined with funds from Colgate University, will provide for a central, on-site DNA sequencing and genotyping facility at Colgate University. The facility will be comprised of an ABI310 Prism Genetic Analyzer (ABI 310) and ancillary equipment including a thermal cycler, a microcentrifuge, and a computer workstation. The facility will allow faculty members, research staff, and students at Colgate University to easily and efficiently carry out DNA sequencing and genotyping for research projects in cell and molecular biology and evolution, systematics, and conservation biology. Specifically, the facility will be used to generate data for the diverse research projects being carried out by the six Major Users on the Colgate University faculty: phylogenetic analysis of annelids and the evolution of larval developmental modes, coevolution of yuccas and yucca moths, large-scale genome rearrangement in ciliated protozoans, spindle pole duplication, regulation of transcription expression in yeast, and the role of carbonic anhydrase in plants. In addition, the equipment will be used by undergraduate students working in collaboration with faculty members and on their own research projects, and Minor Users from nearby Hamilton College will also have access to the facility.