Electronics and Optics for a Fourth Channel for Flow Cytometry

该提案涉及为阿肯色大学费耶特维尔分校购买和安装四色荧光细胞仪所需的电子设备、光学设备和附件。该设备将对现有的FACSort进行改装，该FACSort目前能够进行3色分析。受此影响的项目多种多样。参与这项提议的研究人员来自富布赖特艺术与科学学院和农业学院。将受到最直接影响的项目包括鸟类免疫系统的正常发育；参与运输的蛋白质的诱导；凋亡和抗凋亡信号的分子生物学；以及微生物在高pH值下的生存和分化。该设备将用于细胞、细胞器和孢子的分类和特征以及基因调控。它将提供结合细胞器的大小、复杂性和其他三种颜色来测量新的荧光颜色的可能性。由于绿色荧光蛋白类分子作为报告基因的可用性，使用这些分子开发的新工具将有可能对在极端环境中生存和繁荣的微生物进行新的研究。这些将被用来研究对环境变化的反应。在另一个项目中，潜伏的马立克氏病毒的重新激活将被用作启动死亡和抗死亡信号的工具，用于研究正常的T细胞死亡途径。定义鸡淋巴细胞发育的新抗体将以四种颜色表征，这是目前全面评估哺乳动物B细胞发育所需的最低要求。该仪器将对研究基础设施和学生培训产生重大影响。

9970247Abstract This proposal involves the acquisition and installation of electronics, optics and accessories needed for 4 color fluorescence cytometry for the University of Arkansas, Fayetteville. The equipment will modify an existing FACSort which is currently capable of 3 color analysis. The projects positively impacted by this are quite varied. The investigators involved in this proposal come from both the Fulbright College of Arts and Sciences and the College of Agriculture. The projects that will be most directly affected include normal development in the immune system of birds; induction of the proteins involved in trafficking, molecular biology of apoptotic and anti-apoptotic signals, and microbes living and differentiating at high pH. The equipment will be used for sorting and characterizing cells, organelles and spores and gene regulation. It will provide the potential to measure new fluorescent colors in conjunction with size, complexity of organelles and three other colors. Because of the availability of green fluorescent protein-like molecules as reporter genes, new research into microbes surviving and thriving in extreme environments will be possible using new tools developed using these molecules. These will be used to study responses to environmental shifts. In another project, reactivation of latent Marek's virus will be used as a tool to initiate death and anti-death signals for the study of normal T cell death pathways. New antibodies to define the development of chicken lymphocytes will be characterized in four colors as is the minimum currently required to fully evaluate mammalian B cell development. The instrumentation will impact significantly on both research infrastructure and student training.