Electronics and Optics for a Fourth Channel for Flow Cytometry

摘要本提案涉及阿肯色大学费耶特维尔分校四色荧光细胞仪所需的电子、光学和配件的采购和安装。该设备将改进现有的FACSort，目前能够进行3种颜色分析。受此积极影响的项目各不相同。参与这项提案的研究人员来自富布赖特艺术与科学学院和农业学院。最直接受影响的项目包括鸟类免疫系统的正常发育；该设备将用于细胞、细胞器和孢子的分选和表征以及基因调控。它将提供结合大小、细胞器复杂性和其他三种颜色测量新的荧光颜色的潜力。由于绿色荧光蛋白样分子作为报告基因的可用性，利用利用这些分子开发的新工具，对在极端环境中生存和繁荣的微生物进行新的研究将成为可能。这些将用于研究对环境变化的反应。在另一个项目中，潜伏马立克病毒的再激活将被用作启动死亡和抗死亡信号的工具，用于研究正常T细胞死亡途径。用于确定鸡淋巴细胞发育的新抗体将以四种颜色进行表征，这是目前全面评估哺乳动物B细胞发育所需的最低标准。这些仪器将对研究基础设施和学生培训产生重大影响。

9970247Abstract This proposal involves the acquisition and installation of electronics, optics and accessories needed for 4 color fluorescence cytometry for the University of Arkansas, Fayetteville. The equipment will modify an existing FACSort which is currently capable of 3 color analysis. The projects positively impacted by this are quite varied. The investigators involved in this proposal come from both the Fulbright College of Arts and Sciences and the College of Agriculture. The projects that will be most directly affected include normal development in the immune system of birds; induction of the proteins involved in trafficking, molecular biology of apoptotic and anti-apoptotic signals, and microbes living and differentiating at high pH. The equipment will be used for sorting and characterizing cells, organelles and spores and gene regulation. It will provide the potential to measure new fluorescent colors in conjunction with size, complexity of organelles and three other colors. Because of the availability of green fluorescent protein-like molecules as reporter genes, new research into microbes surviving and thriving in extreme environments will be possible using new tools developed using these molecules. These will be used to study responses to environmental shifts. In another project, reactivation of latent Marek's virus will be used as a tool to initiate death and anti-death signals for the study of normal T cell death pathways. New antibodies to define the development of chicken lymphocytes will be characterized in four colors as is the minimum currently required to fully evaluate mammalian B cell development. The instrumentation will impact significantly on both research infrastructure and student training.