Cellular Microinjection and Fluorescence Imaging System for Analysis of Living Cells

摘要-9970402近年来，生命过程的显微镜技术经历了一次小小的复兴。特别重要的是使用遗传和显微注射荧光探针来跟踪和梳理活细胞和组织中的动态过程。在这笔赠款中，国家科学基金会提供资金购买最先进的显微注射和显微镜成像系统。 该设备将由查尔斯顿学院、南卡罗来纳州医科大学和NOAA东南渔业科学中心的基础科学研究人员使用。这些项目包括应用于活体斑马鱼胚胎中绿色荧光蛋白表达的遗传分析;跟踪组织培养中荧光标记细胞的迁移行为;动态测量发育过程中细胞与细胞之间的通讯;以及荧光跟踪发育中鱼类胚胎中的毒素。使用这种设备的跨学科小组已证明致力于教育，并为年轻人提供机会，让他们在专业研究环境中体验工作。 这些包括从高中到博士后培训的各个层次的学生，特别强调鼓励少数民族参与科学。美国国家科学基金会的这笔赠款将大大有助于南卡罗来纳州查尔斯顿地区的科学研究和教育基础设施。

ABSTRACT - 9970402The microscopy of living processes has undergone a minor renaissance in recent years. Of particular importance has been the use of genetic and microinjected fluorescent probes to track and tease apart dynamic processes in living cells and tissues. In this grant, funds have been provided by the National Science Foundation to purchase a state-of-the-art microinjection and microscope imaging system. This equipment will be used by basic science researchers at the College of Charleston, Medical University of South Carolina and NOAA Southeast Fisheries Science Center. The projects include applications in genetic analysis of green fluorescent protein expression in living zebrafish embryos; following migratory behavior of fluorescently tagged cells in tissue culture; dynamic measurement of cell-to-cell communication in developmental processes; and fluorescent tracking of toxins in developing fish embryos. The interdisciplinary groups using this equipment have proven commitments to education and strong records of providing opportunities for young people to experience work in a professional research environment. These include students at all levels from high school to post-doctoral trainees-with special emphasis on encouraging minority participation in science. This grant by the NSF will contribute significantly to scientific research and education infrastructure in the Charleston, South Carolina area.