Cellular Microinjection and Fluorescence Imaging System for Analysis of Living Cells

用于活细胞分析的细胞显微注射和荧光成像系统

• 批准号: 9970402
• 负责人: Robert Gourdie
• 金额: $ 7.03万
• 依托单位: Medical University of South Carolina
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9970402&HistoricalAwards=false
• 关键词: Cellular; Microinjection; Fluorescence; Imaging; System

ABSTRACT - 9970402The microscopy of living processes has undergone a minor renaissance in recent years. Of particular importance has been the use of genetic and microinjected fluorescent probes to track and tease apart dynamic processes in living cells and tissues. In this grant, funds have been provided by the National Science Foundation to purchase a state-of-the-art microinjection and microscope imaging system. This equipment will be used by basic science researchers at the College of Charleston, Medical University of South Carolina and NOAA Southeast Fisheries Science Center. The projects include applications in genetic analysis of green fluorescent protein expression in living zebrafish embryos; following migratory behavior of fluorescently tagged cells in tissue culture; dynamic measurement of cell-to-cell communication in developmental processes; and fluorescent tracking of toxins in developing fish embryos. The interdisciplinary groups using this equipment have proven commitments to education and strong records of providing opportunities for young people to experience work in a professional research environment. These include students at all levels from high school to post-doctoral trainees-with special emphasis on encouraging minority participation in science. This grant by the NSF will contribute significantly to scientific research and education infrastructure in the Charleston, South Carolina area.

摘要：近年来，生命过程的显微学经历了一次小小的复兴。特别重要的是使用遗传和微注射荧光探针来跟踪和梳理活细胞和组织中的动态过程。在这笔拨款中，国家科学基金会提供了资金，用于购买最先进的显微注射和显微镜成像系统。该设备将由查尔斯顿学院、南卡罗来纳医科大学和NOAA东南渔业科学中心的基础科学研究人员使用。项目包括在斑马鱼活体胚胎绿色荧光蛋白表达的遗传分析中的应用；荧光标记细胞在组织培养中的迁移行为发育过程中细胞间通讯的动态测量以及荧光追踪发育中的鱼类胚胎中的毒素。使用该设备的跨学科小组已经证明了对教育的承诺，并为年轻人提供了在专业研究环境中体验工作的机会。其中包括从高中到博士后培训生的各个层次的学生——特别强调鼓励少数民族参与科学。美国国家科学基金会的这笔拨款将为南卡罗来纳州查尔斯顿地区的科学研究和教育基础设施做出重大贡献。