Fluorescence, Phosphorescence and Excitation Techniques in the Undergraduate Curriculum

本科课程中的荧光、磷光和激发技术

• 批准号: 9972505
• 负责人: Amy Shachter
• 金额: $ 3.36万
• 依托单位: Santa Clara University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9972505&HistoricalAwards=false
• 关键词: Fluorescence; Phosphorescence; Excitation; Techniques; Undergraduate

By using a spectrofluorimeter, students are acquiring skills in fluorescence, phosphorescence and excitation techniques. Twelve specific experiments are being adapted from either the research literature or from the Journal of Chemical Education. In addition, eight new experimental procedures are being developed. The spectrofluorimeter is used in the physical chemistry laboratory to study quenching of excited states and to use selection rules to assign excited states. In the inorganic laboratory, the instrument is used to characterize compounds synthesized in the laboratory, to study the photophysical properties of important excited state complexes, to distinguish between fluorescence and phosphorescence, to follow the progress of a photochemical reaction, and to record a sharp-line excitation spectrum in order to determine ligand field properties. The quantitative determination of trace metal concentrations through the fluorescence of porphyrin complexes is conducted in our analytical course. Protein conformational analysis and concentration determination is investigated by using fluorescence techniques in our biochemistry course. Finally, environmental applications of fluorescence methods are being incorporated into a non-science majors course and general chemistry laboratory. All materials developed are to be available on the internet and presented at chemical education conferences. In addition, several undergraduate research projects involving the spectrofluorimeter are underway. Research results are to be presented by undergraduates at local, regional and national conferences and published with undergraduate co-authors.

通过使用荧光光谱仪，学生掌握了荧光、磷光和激发技术的技能。12个具体的实验改编自研究文献或化学教育杂志。此外，正在开发八种新的实验程序。在物理化学实验室中使用荧光光谱仪来研究激发态的猝灭，并使用选择规则来分配激发态。在无机实验室中，该仪器用于对实验室合成的化合物进行表征，研究重要激发态配合物的光物理性质，区分荧光和磷光，跟踪光化学反应的进展，记录锐线激发光谱以确定配体场性质。在我们的分析课程中，利用卟啉配合物的荧光定量测定痕量金属的浓度。在生物化学课程中，利用荧光技术对蛋白质的构象分析和浓度测定进行了研究。最后，荧光方法的环境应用被纳入非科学专业的课程和普通化学实验室。所有开发的材料都可以在互联网上获得，并在化学教育会议上展示。此外，几个涉及荧光光谱仪的本科生研究项目正在进行中。研究成果将由本科生在地方、区域和国家会议上发表，并与本科生共同发表。