Fluorescence, Phosphorescence and Excitation Techniques in the Undergraduate Curriculum

通过使用荧光分光光度计，学生获得荧光、磷光和激发技术方面的技能。12个具体的实验要么来自研究文献，要么来自《化学教育杂志》。此外，正在开发八种新的实验程序。在物理化学实验室中，荧光光谱仪用于研究激发态的猝灭，并使用选择规则来指定激发态。在无机实验室中，该仪器用于表征实验室合成的化合物，研究重要激发态配合物的光物理性质，区分荧光和磷光，跟踪光化学反应的进程，并记录锐线激发光谱，以确定配位场性质。在我们的分析过程中，通过卟啉配合物的荧光定量测定了痕量金属的浓度。在我们的生物化学课程中，利用荧光技术研究了蛋白质的构象分析和浓度测定。最后，荧光方法的环境应用正在被纳入非理科专业的课程和普通化学实验室。所有开发的材料都将在互联网上获得，并在化学教育会议上展示。此外，几个涉及荧光分光计的本科生研究项目正在进行中。研究成果将由本科生在地方、地区和国家会议上发表，并与本科生合著者一起发表。

By using a spectrofluorimeter, students are acquiring skills in fluorescence, phosphorescence and excitation techniques. Twelve specific experiments are being adapted from either the research literature or from the Journal of Chemical Education. In addition, eight new experimental procedures are being developed. The spectrofluorimeter is used in the physical chemistry laboratory to study quenching of excited states and to use selection rules to assign excited states. In the inorganic laboratory, the instrument is used to characterize compounds synthesized in the laboratory, to study the photophysical properties of important excited state complexes, to distinguish between fluorescence and phosphorescence, to follow the progress of a photochemical reaction, and to record a sharp-line excitation spectrum in order to determine ligand field properties. The quantitative determination of trace metal concentrations through the fluorescence of porphyrin complexes is conducted in our analytical course. Protein conformational analysis and concentration determination is investigated by using fluorescence techniques in our biochemistry course. Finally, environmental applications of fluorescence methods are being incorporated into a non-science majors course and general chemistry laboratory. All materials developed are to be available on the internet and presented at chemical education conferences. In addition, several undergraduate research projects involving the spectrofluorimeter are underway. Research results are to be presented by undergraduates at local, regional and national conferences and published with undergraduate co-authors.