Extremely Halophilic Bacteria: A Remarkably Tractable System for Teaching Molecular Techniques

极嗜盐细菌：一种非常易于处理的分子技术教学系统

• 批准号: 9981129
• 负责人: Richard Shand
• 金额: $ 7.18万
• 依托单位: Northern Arizona University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9981129&HistoricalAwards=false
• 关键词: Extremely; Halophilic; Bacteria; Remarkably; Tractable

Biological Science (61) This project adapts the unique and tractable ecology, genetics, physiology and biochemistry of an extremely halophilic (i.e., "salt loving") bacterium to a junior level teaching laboratory in molecular techniques. It is based on pedagogical techniques outlined by the Johnsons (Johnson, D. W. and R.T. Johnson, 1993, Cooperative Learning and College Teaching Newsletter, 3:6-9), further detailed by Herreid (Herreid, C.F., 1998, BioScience 48:553-559).This project accomplishes four objectives: 1) students are made aware of the importance and relevance of the ecology of the group of organisms used as models in the molecular techniques laboratory; 2) students learn that biology is a quantitative science, not solely an observational one; 3) all students participate equally in an authentic "hands-on" molecular biology laboratory; and 4) students are prepared for more sophisticated molecular experiments in an advanced senior level course and in independent, undergraduate research projects. Students and faculty whose majors and backgrounds are traditionally not considered "molecular" in nature are welcomed and encouraged to enroll in the course. In addition, a laboratory manual (both hard copy and Web-based) is being produced and disseminated, and bacterial strains and environmental samples are supplied for the asking.

生物科学（61）该项目采用了独特的和易于处理的生态学，遗传学，生理学和生物化学的极端嗜盐（即，“嗜盐”的细菌的分子技术初级教学实验室。 它是基于教学技术概述的约翰逊（约翰逊，D。W.和R.T.约翰逊，1993年，合作学习和大学教学通讯，3：6-9），进一步详细介绍了赫雷德（赫雷德，C.F.，1998，BioScience 48：553-559）。该项目实现了四个目标：1）使学生意识到在分子技术实验室中用作模型的生物群体的生态学的重要性和相关性; 2）学生学习生物学是一门定量科学，而不仅仅是一门观察科学; 3）所有学生平等地参与真正的“动手”分子生物学实验室;以及4）学生在高级高级课程和独立的本科研究项目中为更复杂的分子实验做好准备。 学生和教师的专业和背景传统上不被认为是“分子”的性质是欢迎和鼓励报名参加课程。 此外，正在制作和分发一份实验室手册（包括硬拷贝和网络版），并应要求提供细菌菌株和环境样本。