Generic technology for halophilic bacteria using genomic data from next genearation sequencer

利用来自突尼斯不同地区的分离菌株，研究了中等嗜盐细菌的通用技术，以便应用于盐度条件下的生物修复。研究了嗜盐菌的筛选条件、下一代测序仪的基因组分析、转化质粒载体构建、基因干扰物文库构建以及石油相关化合物降解基因的收集。目前正在利用上述材料构建在盐度条件下工作的石油降解细菌。

Generic technology for moderately halophilic bacteria was examined using isolates from various area of Tunisia in order to apply for bioremediation under salinity conditions. Screening conditions for the variety of halophiles, genome analysis using next generation sequencer, plasmid vector construction with transformation, gene disruptant library construction, and collection of petroleum related compounds degradative genes were examined. Construction of petroleum degradation bacteria working under a salinity condition using the above materials is under progress.