Icosahedral Virion Scaffolding Proteins

二十面体病毒颗粒支架蛋白

• 批准号: 9982284
• 负责人: Bentley Fane
• 金额: $ 31.1万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-15 至 2003-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9982284&HistoricalAwards=false
• 关键词: Icosahedral; Virion; Scaffolding; Proteins

9982284FaneVirion morphogenesis is often dependent on viral scaffolding proteins. Analogous to scaffoldings used in building construction, these proteins direct the assembly of viral components into infectious agents, but are not found in the final product. Different regions, or domains, within scaffolding proteins, and all proteins in general, perform different functions. And with genetic engineering, it is possible to determine the functions of these domains. In these studies, the functional domains of the Microviridae scaffolding proteins are elucidated in experiments conducted with chimeric proteins. These chimeras are constructed from protein sequences derived from different, but evolutionarily related viruses. Some chimeric proteins can only perform a subset of the functions performed by the naturally occurring proteins. When these partially functional proteins interact within other viral components they can inhibit morphogenesis, preventing virus replication. In turn, viruses can mutate to become resistant to the inhibitory effects of the genetically engineered proteins. It is expected that the results of these studies will provide insights into the design of recombinant proteins, strategies for possible antiviral agents, mechanisms of resistance to antiviral agents and virion morphogenesis.

9982284 FaneVirion形态发生通常依赖于病毒支架蛋白。类似于建筑中使用的支架，这些蛋白质指导病毒成分组装成感染因子，但在最终产品中没有发现。支架蛋白和所有蛋白质中的不同区域或结构域通常执行不同的功能。通过基因工程，可以确定这些结构域的功能。在这些研究中，在用嵌合蛋白进行的实验中阐明了微病毒科支架蛋白的功能结构域。这些嵌合体是由来自不同但进化上相关的病毒的蛋白质序列构建的。一些嵌合蛋白质只能执行由天然存在的蛋白质执行的功能的子集。当这些部分功能性蛋白质与其他病毒成分相互作用时，它们可以抑制形态发生，阻止病毒复制。反过来，病毒可以突变，对基因工程蛋白质的抑制作用产生抗性。预计这些研究的结果将为重组蛋白的设计、可能的抗病毒药物的策略、抗病毒药物的抗性机制和病毒体形态发生提供见解。