Purchase of a Differential Scanning Calorimeter for Studies of DNA Polymerases

购买差示扫描量热计用于 DNA 聚合酶研究

• 批准号: 9982357
• 负责人: Brian Hales
• 金额: $ 7.33万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-15 至 2003-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9982357&HistoricalAwards=false
• 关键词: Purchase; Differential; Scanning; Calorimeter; Studies

This award from the Chemistry Research Instrumentation and Facilities (CRIF) Program will enable the Department of Chemistry at Louisiana State University and A&M College to purchase a differential scanning calorimeter. This equipment will be used primarily by junior faculty member Vincent J. LiCata, whose research focuses on studies of DNA polymerases. He will carry out parallel quantitative measurements of the thermodynamics of unfolding of four DNA polymerases from bacteria. In addition, Dr. LiCata will measure the pH, water activity, and ionic dependencies of unfolding of these polymerases. This project will provide the first calorimetric determinations of the stability of any of these polymerases. One of the polymerases Dr. LiCata will study remains almost completely uncharacterized biophysically, yet it is the most commonly used polymerase in polymerase chain reaction, or PCR. PCR enables scientists to mass-produce DNA molecules from incredibly small amounts of material; thus it is useful to a wide range of scientists, from forensic scientists to those working on the human genome project.

化学研究仪器和设施（CRIF）计划的这一奖项将使路易斯安那州立大学和A M学院的化学系能够购买差示扫描量热仪。 该设备将主要由初级教员Vincent J. LiCata使用，他的研究重点是DNA聚合酶的研究。 他将对细菌中四种DNA聚合酶展开的热力学进行平行定量测量。 此外，LiCata博士还将测量这些聚合酶解折叠的pH值、水活度和离子依赖性。 该项目将提供任何这些聚合酶的稳定性的第一个量热测定。 LiCata博士将要研究的一种聚合酶几乎完全没有生物特征，但它是聚合酶链反应（PCR）中最常用的聚合酶。 PCR使科学家能够从极少量的材料中大量生产DNA分子;因此它对从法医科学家到人类基因组计划工作人员的广泛科学家都很有用。