Analysis of Arabidopsis PRP-Cell Wall Interactions

拟南芥 PRP-细胞壁相互作用分析

• 批准号: 9982749
• 负责人: Mary Tierney
• 金额: $ 15万
• 依托单位: University of Vermont & State Agricultural College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9982749&HistoricalAwards=false
• 关键词: Analysis; Arabidopsis; PRP; Cell; Wall

Arabidopsis thaliana provides a model system in which both molecular genetic and cell biology approaches can be used to evaluate and define the function of structural proteins in the plant cell wall. Proline rich proteins (PRPs) represent afamily of structural cell wall proteins which have been implicated as contributing to maintenance of cell function and in generalized defense mechanisms in response to plant stress. Transgenic plants expressing a tagged version of a PRP will be used to 1) explore the pattern of cell wall deposition for this protein during plant development; and 2) to examine the ability of this protein to become crosslinked within walls of different cell types. Mutants altered in the development of cell types associated with the expression of this protein will also be characterized to determine if changes in cell shape and function observed in these mutants can disrupt or alter normal PRP/wall interactions. Using a similar approach, several other members of the Arabidopsis PRP(AtPRP) gene family will be analyzed to determine if individual gene family members make unique contributions to extracellular matrix structure in specific cell types. A PCR-based screen to identify potential T-DNA insertion mutants for several members of the AtPRP gene family will be initiated. Mutants identified from these screens will be characterized at a molecular level and, if time permits, will be analyzed for phenotypes associated with the loss of AtPRP expression.

拟南芥提供了一个模型系统，其中分子遗传学和细胞生物学方法可以用来评估和定义植物细胞壁中的结构蛋白的功能。 富含脯氨酸的蛋白质（Proline rich protein，PRPs）是一类结构性细胞壁蛋白质，它们参与维持细胞功能和植物对逆境的防御机制。 表达标记形式的PRP的转基因植物将用于1）探索植物发育期间该蛋白质的细胞壁沉积模式;和2）检查该蛋白质在不同细胞类型的壁内交联的能力。 还将表征在与该蛋白表达相关的细胞类型发育中改变的突变体，以确定在这些突变体中观察到的细胞形状和功能的变化是否可以破坏或改变正常的PRP/壁相互作用。 使用类似的方法，将分析拟南芥PRP（AtPRP）基因家族的其他几个成员，以确定单个基因家族成员是否对特定细胞类型中的细胞外基质结构做出独特的贡献。 将启动基于PCR的筛选，以确定AtPRP基因家族几个成员的潜在T-DNA插入突变体。 从这些筛选中鉴定的突变体将在分子水平上进行表征，如果时间允许，将分析与AtPRP表达缺失相关的表型。