Molecular Analysis of PRP Insolubilization and Wound-inducedExpression

植物细胞壁是重要的细胞结构， 植物生长过程中的许多生物和物理作用。 这 结构是动态的，随着增长而变化， 细胞分化和与环境的相互作用。 拟议研究的长期目标是确定 富含脯氨酸的细胞壁蛋白（PRPs）在植物生长过程中的作用 增长和发展，并确定负责的因素 控制表达。 PRP在特定细胞中表达 类型，并在特定的植物组织中由创伤诱导。 在 此外，这些蛋白质在细胞壁内交联， 这一过程似乎是由物理损伤刺激的。 然而，转录调控所需的序列 这些基因或氨基酸的重要性， 细胞壁内的不溶作用知之甚少。 这个奖项的具体目标，以确定特定的区域 在SbPRP 1和SbPRP 2启动子中， 这些基因在大豆中的创伤诱导表达， 确定氨基酸发挥关键作用， 这些蛋白质在细胞壁内的不溶解。 使用 启动子/GUS在稳定转化植物中的表达将允许PI 区分调节表达的序列， 这些基因在发育过程中和对身体损伤的反应。 另外，蒂尔尼博士将能够研究 受伤组织内的类型能够对伤口做出反应- 诱导信号，最终导致SbPRP 1和2表达。 她还将研究特定的氨基酸， 对于这些蛋白质在细胞内的不溶解是重要的 墙 “合成”PRP的使用将使得有可能 鉴定能够在细胞内形成交联的氨基酸 壁，所涉及的相互作用的类型，以及这些氨基 酸对于这些蛋白质的不溶解是重要的， 细胞类型。 这些研究还将描述基质， 参与细胞壁基质形成的关键酶， 作为鉴定这些蛋白质的起点。

The plant cell wall is an important cellular structure that has numerous biological and physical roles during plant growth. This structure is dynamic and changes as a consequence of growth, cellular differentiation and interactions with the environment. The long term goals of the proposed research are to determine the function of proline-rich cell wall proteins (PRPs) during plant growth and development and to identify the factors responsible for their controlled expresion. PRPs are expressed in specific cell types and are induced by wounding in a specific plant tissues. In addition, these proteins become cross-linked within the cell wall, a process which appears to be stimulated by physical damage. However, the sequences required for the transcriptional regulation of these genes or the amino acids important for their insolubilization within the cell wall are poorly understood. The specific goals of this award to identify specific regions within the SbPRP1 and SbPRP2 promoters which are required for the wound-inducible expression of these genes in soybean and to identify amino acids which play a critical role in the insolubilization of these proteins within the cell wall. The use of promoter/GUS in stably transformed plants will allow PIs to distinguish between sequences which regulate the expression of these genes during development and in response to physical damage. In addition, Dr. Tierney will be able to investigate which cell types within wounded tissue are capable of responding to the wound- induced signals which ultimately result in SbPRP1 and 2 expression. She will also investigate the specific amino acids which are important for the insolubilzation of these proteins within the cell wall. The use of "synthetic" PRPs will make it possible to identify amino acids capable of forming cross-links within the cell wall, the types of interactions involved, and whether these amino acids are important for insolubilization of these proteins in all cell types. These studies will also describe the substrates for key enzymes involved in cell wall matrix formation and will serve as a starting point for the identification of these proteins.