Characterization of the diazotroph communities in the Subtropical North Atlantic (M60/5)

北大西洋副热带固氮微生物群落的特征 (M60/5)

• 批准号: 13922303
• 负责人: Professorin Dr. Julie La Roche, Ph.D.
• 金额: --
• 依托单位: Department of Oceanography
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/13922303?language=en
• 关键词: Characterization; diazotroph; communities; Subtropical; North

The importance of nitrogen fixation in the marine nitrogen cycle has been underestimated until recently (Gruber and Sarmiento, 1997). One of the fundamental questions in assessing the importance of nitrogen fixation is the paucity of information concerning the diversity and activity of marine diazotrophs in the world s ocean. During the Meteor 60/5 cruise, we collected samples for the analysis of nifH gene presence and expression. Using Taqman probes specific for various diazotrophs phylotypes present in our North Atlantic samples, we propose here to analyze the Meteor 60 samples to obtain quantitative data from nutrient addition bioassay experiments. In addition, we propose to characterize the dominant 16 S and 18 S rDNA phylotypes of the planktonic microbial community present during these incubations. The bacterioplankton phylotypes will be characterized both by TRFLP analysis of 16S rDNA while the eukaryotic component will be characterized mainly by cloning and sequencing of the 18 S rDNA. The clarity of the response from the bioassay experiments conducted during the Meteor 60/5 justifies a more complete analysis of the microbial community structure in the various treatments, using molecular techniques.

直到最近，人们一直低估了固氮在海洋氮循环中的重要性（Gruber和Sarmiento，1997年）。评估固氮作用重要性的一个基本问题是缺乏有关世界海洋中海洋固氮生物多样性和活动的信息。在Meteor 60/5巡航期间，我们收集了用于分析nifH基因存在和表达的样本。使用Taqman探针特定的各种固氮生物的类型存在于我们的北大西洋样品，我们建议在这里分析流星60样品，以获得定量数据，从营养素添加生物测定实验。此外，我们建议的特点，占主导地位的16 S和18 S rDNA的细菌群落在这些孵育过程中存在的。浮游细菌浮游类型的特征将通过16 S rDNA的TRFLP分析，而真核成分的特征将主要通过18 S rDNA的克隆和测序。在Meteor 60/5期间进行的生物测定实验的反应清晰，证明了使用分子技术对各种处理中的微生物群落结构进行更全面分析的合理性。

项目成果

Iron limits primary productivity during spring bloom development in the central North Atlantic

• DOI: 10.1111/j.1365-2486.2006.01122.x
• 发表时间: 2006-04-01
• 期刊: GLOBAL CHANGE BIOLOGY
• 影响因子: 11.6
• 作者: Moore, CM;Mills, MM;La Roche, J
• 通讯作者: La Roche, J

Database of diazotrophs in global ocean: abundance, biomass and nitrogen fixation rates

• DOI: 10.5194/essd-4-47-2012
• 发表时间: 2012-01-01
• 期刊: EARTH SYSTEM SCIENCE DATA
• 影响因子: 11.4
• 作者: Luo, Y. -W.;Doney, S. C.;Zehr, J. P.
• 通讯作者: Zehr, J. P.

Diazotrophic bacteria respond to Saharan dust additions

• DOI: 10.3354/meps10109
• 发表时间: 2012-01-01
• 期刊: MARINE ECOLOGY PROGRESS SERIES
• 影响因子: 2.5
• 作者: Langlois, Rebecca J.;Mills, Matthew M.;LaRoche, Julie
• 通讯作者: LaRoche, Julie