U.S.-Sweden Cooperative Research: Cell Biology and Enzyme Localization Research in the Marine Diazotroph, Trichodesmium

美国-瑞典合作研究：海洋固氮菌、毛藻的细胞生物学和酶定位研究

• 批准号: 9014842
• 负责人: Edward Carpenter
• 金额: $ 1.66万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9014842&HistoricalAwards=false
• 关键词: U.S.; Sweden; Cooperative; Research; Cell

This award will support collaborative research in biological oceanography between Dr. Edward Carpenter, State University of New York at Stony Brook, and Dr. Birgitta Bergman, Institute of Physiological Botany, University of Uppsala, Sweden. The objective of the proposed research is to study the physiology of nitrogen fixation in the pelagic cyanobacterium Trichodesmium. This organism is significant in the biology of the world's oceans as a major contributor of new nitrogen to tropical and subtropical seas. In the proposed project, Dr. Carpenter will study advanced techniques on enzyme localization, molecular biology and electron microscopy in order to address major unresolved questions concerning this enigmatic organism, in particular, how this non-heterocystous colony is able to fix nitrogen during the day under aerobic conditions. He will focus on: 1) the location and abundance of the enzyme nitrogenase through diel cycles, 2) the localization and abundance of N transforming enzymes GS and GOGAT, with the goal of understanding how fixed nitrogen is transferred from diazotrophic cells to vegetative cells, 3) the location of oxygen protective enzymes, such as uptake hydrogenases and superoxide dismutase, and 4) the location of other proteins involved in photosynthesis. The two major Trichodesmium species, erythraeum and thiebautii, will be contrasted. The research will be carried out through the study of Trichodesmium samples at the Institute of Physiological Botany in Uppsala, jointly with Dr. Bergman. The complementary expertise of the two investigators in immuno-localization at the LM and TEM level of key proteins in cynobacteria will contribute to the success of the proposed project.

该奖项将支持纽约州立大学石溪分校的Edward Carpenter博士和瑞典乌普萨拉大学生理植物学研究所的Birgitta Bergman博士在生物海洋学方面的合作研究。本研究的目的是研究远洋蓝藻毛霉菌的固氮生理。作为热带和亚热带海洋新氮的主要贡献者，这种生物在世界海洋生物学中具有重要意义。在拟议的项目中，卡彭特博士将研究酶定位、分子生物学和电子显微镜方面的先进技术，以解决关于这种神秘生物的主要悬而未决的问题，特别是这种非异质晶体菌落如何能够在有氧条件下在白天固定氮。他将专注于：1)通过Diel循环的固氮酶的位置和丰度，2)N转换酶GS和GOGAT的定位和丰度，目的是了解固定氮是如何从重氮营养细胞转移到营养细胞的，3)氧保护酶的位置，如吸收氢酶和超氧化物歧化酶，以及4)参与光合作用的其他蛋白质的位置。两个主要的毛霉物种，红曲霉和蒂鲍蒂菌，将被对比。这项研究将通过乌普萨拉生理植物学研究所的毛霉菌样本研究，与伯格曼博士共同进行。这两位研究人员在共生细菌关键蛋白的光镜和透射电子显微镜水平上的免疫定位方面的互补专业知识将有助于拟议项目的成功。