Automated DNA Sequencer for Microbial Ecology Studies

用于微生物生态学研究的自动 DNA 测序仪

• 批准号: 0070139
• 负责人: John Gunderson
• 金额: $ 5.45万
• 依托单位: Tennessee Technological University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070139&HistoricalAwards=false
• 关键词: Automated; DNA; Sequencer; Microbial; Ecology

Abstract 0070139 (Automated DNA Sequencer for Microbial Ecology Studies) GundersonA LICOR 4200L2 automated DNA sequencer will be used for microbial ecology studies being conducted at TennesseeTechnological University. An interdisciplinary nucleus of faculty and students interested in molecular microbial ecology has developed here, with representatives from the Department of Biology, the Center for the Management, Utilization and Protection of Water Resources, and the Department of Civil Engineering engaged in funded research in the area of microbial ecology. The research activities have grown beyond a level which can be supported by our manual sequencing facilities.The research projects that would immediately employ an automated sequencer include an rRNA-based survey of the distribution of parasitic dinoflagellates in Chesapeake Bay, the analysis of protistan food vacuole contents, the determination of the identity of prokaryotic symbionts of termite flagellates, the screening of natural and man-made habitats for the presence ofLegionella-like amoebal pathogens, and the determination of the effects of pesticides on prokaryotic communities living in constructed wetlands. Although microbial ecologists would be the primary users of the machine, other faculty members would also use an automated sequencer if one were available.An automated DNA sequencer would facilitate the ongoing research of faculty members at TTU, help provide necessary infrastructural support to a new Ph.D. program in environmental sciences, and allow both graduate and undergraduate students to be involved in DNA sequencing projects. It's presence would also eliminate the risk and inconvenience of radioisotope use.

摘要0070139(用于微生物生态研究的自动DNA测序仪)Gunderson田纳西理工大学正在进行的微生物生态学研究将使用LICOR 4200L2自动DNA测序仪。这里已经形成了一个由对分子微生物生态学感兴趣的教职员工和学生组成的跨学科核心，生物系、水资源管理、利用和保护中心以及土木工程系的代表参与了微生物生态学领域的资助研究。研究活动已经发展到我们的人工测序设施可以支持的水平。立即使用自动测序仪的研究项目包括基于rRNA的切萨皮克湾寄生甲藻分布的调查、原生动物食物液泡内容物的分析、白蚁鞭毛原核共生生物的鉴定、筛选天然和人造生境中存在军团菌的阿米巴病原体，以及确定杀虫剂对人工湿地中原核生物群落的影响。虽然微生物生态学家将是这台机器的主要用户，但其他教职员工也会使用自动测序仪，如果有的话。自动DNA测序仪将促进TTU教职员工正在进行的研究，帮助为环境科学的新博士项目提供必要的基础设施支持，并允许研究生和本科生参与DNA测序项目。它的存在还将消除放射性同位素使用的风险和不便。