An Automated DNA Sequencer for the Claremont Colleges

克莱蒙特学院的自动 DNA 测序仪

• 批准号: 0070377
• 负责人: Elizabeth Friar
• 金额: --
• 依托单位: Rancho Santa Ana Botanic Garden
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2001-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070377&HistoricalAwards=false
• 关键词: Automated; DNA; Sequencer; Claremont; Colleges

0070377Elizabeth A. FriarRancho Santa Ana Botanical GardensAn Automated DNA Sequencer for the Claremont CollegesThis award will allow Rancho Santa Ana Botanic Garden (RSABG) to purchase an ABI Prism 377 DNA sequencer (with an XL-C upgrade) for use in the core sequencing laboratory that it provides for use by biologists at the Claremont Colleges. A highly reliable and accurate instrument, the ABI 377 will be used in a core sequencing lab to support research by six major users and a number of minor users at RSABG, Pomona College and Harvey Mudd College. Added to an existing ABI 373 currently operated by RSABG, the ABI 377 will increase overall lab productivity by at least ten-fold, allowing the laboratory to meet a rapidly growing demand for additional sequencing capability for a number of projects. The primary research projects are 1) population and conservation genetics of the endangered and endemic Hawaiian silversword alliance; 2) examination of reticulate patterns of diversification within Polemoniaceae; 3) evolution and classification of North American chloridoid grasses; 4) determinants of homing endonuclease I-Cre I function; 5) molecular phylogeny of hydra and construction of a molecular phylogeny of terrestrial Isopods; and 6) use of DNA sequence data to infer evolutionary relationships among Alcyonacean soft corals. The sequencer will also significantly strengthen education and training activities for graduate and undergraduate students in systematics, evolution, and molecular biology.

0070377伊丽莎白A. FriarRancho圣安娜植物园克莱蒙特学院的自动DNA测序仪该奖项将允许Rancho圣安娜植物园（RSABG）购买ABI Prism 377 DNA测序仪（带有XL-C升级），用于核心测序实验室，该实验室提供给克莱蒙学院的生物学家使用。ABI 377是一种高度可靠和准确的仪器，将用于核心测序实验室，以支持RSABG、波莫纳学院和哈维穆德学院的六个主要用户和一些次要用户的研究。添加到RSABG目前运行的现有ABI 373上，ABI 377将使实验室的整体生产力提高至少10倍，使实验室能够满足许多项目对额外测序能力的快速增长的需求。主要研究项目有：1）夏威夷银剑属濒危和特有植物的种群和保护遗传学; 2）花葵科植物多样性的网状模式研究; 3）北美绿球藻的进化和分类; 4）归巢核酸内切酶I-Cre I功能的决定因素; 5）水螅的分子遗传学和陆生等足类动物分子遗传学的构建;以及6）利用DNA序列数据推断软珊瑚类之间的进化关系。测序仪还将大大加强系统学、进化和分子生物学研究生和本科生的教育和培训活动。