An Automated DNA Sequencer for the Claremont Colleges

0070377Elizabeth A. FriarRancho Santa Ana植物园Claremont学院自动DNA测序仪该合同将允许Rancho Santa Ana植物园（RSABG）购买ABI Prism 377 DNA测序仪（带有xml - c升级），用于其提供给Claremont学院生物学家使用的核心测序实验室。ABI 377是一种高度可靠和精确的仪器，将用于核心测序实验室，以支持RSABG， Pomona学院和Harvey Mudd学院的六个主要用户和一些次要用户的研究。与目前由RSABG运营的ABI 373相比，ABI 377将使实验室的整体生产力提高至少10倍，使实验室能够满足许多项目对额外测序能力快速增长的需求。主要研究项目有：1)濒危和特有夏威夷银剑种群的种群和保护遗传学；2)蓼科植物多样化的网状格局研究；3)北美类氯草的进化与分类；4)归巢内切酶I- cre - I功能的决定因素；5)水螅类分子系统发育与陆生等足类分子系统发育的构建；6)利用DNA序列数据推断藻科软珊瑚的进化关系。该测序仪还将大大加强研究生和本科生在系统学、进化和分子生物学方面的教育和培训活动。

0070377Elizabeth A. FriarRancho Santa Ana Botanical GardensAn Automated DNA Sequencer for the Claremont CollegesThis award will allow Rancho Santa Ana Botanic Garden (RSABG) to purchase an ABI Prism 377 DNA sequencer (with an XL-C upgrade) for use in the core sequencing laboratory that it provides for use by biologists at the Claremont Colleges. A highly reliable and accurate instrument, the ABI 377 will be used in a core sequencing lab to support research by six major users and a number of minor users at RSABG, Pomona College and Harvey Mudd College. Added to an existing ABI 373 currently operated by RSABG, the ABI 377 will increase overall lab productivity by at least ten-fold, allowing the laboratory to meet a rapidly growing demand for additional sequencing capability for a number of projects. The primary research projects are 1) population and conservation genetics of the endangered and endemic Hawaiian silversword alliance; 2) examination of reticulate patterns of diversification within Polemoniaceae; 3) evolution and classification of North American chloridoid grasses; 4) determinants of homing endonuclease I-Cre I function; 5) molecular phylogeny of hydra and construction of a molecular phylogeny of terrestrial Isopods; and 6) use of DNA sequence data to infer evolutionary relationships among Alcyonacean soft corals. The sequencer will also significantly strengthen education and training activities for graduate and undergraduate students in systematics, evolution, and molecular biology.