An Automated DNA Sequencer for the Department of Biological Sciences, University of Alabama

阿拉巴马大学生物科学系的自动 DNA 测序仪

• 批准号: 0070351
• 负责人: Charles Lydeard
• 金额: $ 5.22万
• 依托单位: University of Alabama Tuscaloosa
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070351&HistoricalAwards=false
• 关键词: Automated; DNA; Sequencer; Department; Biological

Award abstract - DBI-0070351"An Automated DNA Sequencer for the Department of Biological Sciences, The University of Alabama"Accumulating genomic data provides an opportunity to examine evolutionary relationships within a wide variety of aquatic species. On going phylogenetic studies of snails, bivalves, sunfishes, basses, and zoosporic fungi provide a wealth of information on the ecology, biogeography, and evolution of morphological and life-history features of these diverse groups. An automated DNA sequencer will be used to conduct genomic studies more efficiently and accurately on vertebrates, invertebrates, and microorganisms. The specific equipment to be used is the LI-COR 4200S-2 Gene ReadIR DNA Analysis System. The LI-COR system produces high quality raw image data by electrophoretically separating labeled DNA and automatically scanning gels for fluorescence at two infrared (IR) wavelengths. LI-COR produces high accuracy reads and eliminates the need for radioactive labeling. In addition to DNA sequencing, the LI-COR system has hardware and software capabilities for microsatellite DNA research. In addition to increasing research productivity and output, this equipment will provide excellent training opportunities for students with an interest in biotechnology and genomic research. Virtually all DNA sequencing is conducted today with an automated sequencer. Having the equipment in the Biology Department will provide valuable hands-on training and increase the competitiveness and knowledge base of graduate and undergraduate biology students at the University of Alabama.

奖励摘要- DBI-0070351“阿拉巴马大学生物科学系的自动DNA测序仪”积累基因组数据提供了一个机会来检查各种水生物种的进化关系。正在进行的蜗牛、双壳类、太阳鱼、底栖和游动孢子真菌的系统发育研究为这些不同类群的生态学、生物地理学、形态进化和生活史特征提供了丰富的信息。自动DNA测序仪将用于更有效、更准确地对脊椎动物、无脊椎动物和微生物进行基因组研究。要使用的具体设备是LI-COR 4200S-2基因读取DNA分析系统。LI-COR系统通过电泳分离标记的DNA和自动扫描凝胶在两个红外（IR）波长的荧光来产生高质量的原始图像数据。LI-COR产生高精度读数，消除了放射性标记的需要。除了DNA测序外，LI-COR系统还具有用于微卫星DNA研究的硬件和软件功能。除了提高研究生产力和产出外，该设备还将为对生物技术和基因组研究感兴趣的学生提供极好的培训机会。如今，几乎所有的DNA测序都是用自动测序仪进行的。在生物系拥有这些设备将提供宝贵的实践培训，并增加阿拉巴马大学生物研究生和本科生的竞争力和知识基础。