Instrumentation for Plant Cell and Molecular Biology

植物细胞和分子生物学仪器

• 批准号: 0070387
• 负责人: Marvin Fawley
• 金额: $ 12.13万
• 依托单位: North Dakota State University Fargo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-15 至 2002-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070387&HistoricalAwards=false
• 关键词: Instrumentation; Plant; Cell; Molecular; Biology

Abstract Fawley Modern instrumentation in support of research in plant cellular and molecular biology will be housed in the Department of Botany/Biology, North Dakota State University, Fargo. Specifically, a research microscope, a high-speed centrifuge, an ultracentrifuge, rotors, a scintillation counter, and culture equipment will be used in a variety of botanical studies. Four researchers will be the primary users of this equipment. Dr. Marvin Fawley is investigating the phylogeny of green algae and the molecular diversity of coccoid green algae. The equipment will support efforts to describe new species of coccoid green algae, and continuing investigations of coccoid green algal diversity. Dr. Karen Phillips is studying phytoplankton communities and population dynamics using molecular techniques and light microscopy. For this project, her work will focus primarily on winter phytoplankton communitiesand population dynamics of freshwater coccoid green algae. Dr. Marc Anderson is studying the influence of environmental stress on metabolic processes in plants. Dr. Anderson will investigate the response of maize seedlings to chilling-induced oxidative stress, specifically focusing on processes in the mitochondria. Dr. Alan White is investigating the biosynthesis of cell wall polysaccharides in plant Golgi membranes. This equipment will support his efforts to purify enzymes involved in the synthesis of xylogulcans in the Golgi, specifically xyloglucan glucosyltransferase and xyloglucan xylosyltransferase. The results of this work will have significant impact on several areas of plant biology. Work on coccoid algae and phytoplankton population dynamics will help revise old dogmas on the diversity of coccoid algae and the dynamics of phytoplankton under the ice in winter. Research will also lead to an understanding of the mechanism(s) (e.g. induced transcription, protein modification, etc.) by which enzyme activities are enhanced during chilling stress. Finally, an increased understanding of xyloglucan biosynthesis will add significantly to our understanding of cell wall polysaccharides, the process of cell wall synthesis, and the structure and functional organization of the plant Golgi apparatus. Biology students and other investigators will have full access to this equipment for their research and training

支持植物细胞和分子生物学研究的现代仪器将设在法戈的北达科他州州立大学植物学/生物学系。具体来说，研究显微镜，高速离心机，超离心，转子，闪烁计数器和培养设备将用于各种植物学研究。四名研究人员将是该设备的主要用户。Marvin Fawley博士正在研究绿色藻类的繁殖和球形绿色藻类的分子多样性。 该设备将支持描述球形绿色藻类新物种的工作，并支持继续调查球形绿色藻类的多样性。 凯伦·菲利普斯博士正在利用分子技术和光学显微镜研究浮游植物群落和种群动态。 在这个项目中，她的工作将主要集中在冬季浮游植物群落和淡水球状绿色藻类的种群动态。 马克安德森博士正在研究环境压力对植物新陈代谢过程的影响。 安德森博士将研究玉米幼苗对低温诱导的氧化应激的反应，特别关注线粒体中的过程。 艾伦白色博士正在研究植物高尔基体膜中细胞壁多糖的生物合成。 该设备将支持他的努力，以纯化参与高尔基体中木葡聚糖合成的酶，特别是木葡聚糖葡糖基转移酶和木葡聚糖木糖基转移酶。这项工作的结果将对植物生物学的几个领域产生重大影响。 对球藻和浮游植物种群动态的研究将有助于修正关于球藻多样性和冬季冰下浮游植物动态的旧教条。 研究还将导致对机制的理解（例如诱导转录，蛋白质修饰等）。 低温胁迫下酶活性增强。 最后，对木葡聚糖生物合成的了解将大大增加我们对细胞壁多糖、细胞壁合成过程以及植物高尔基体的结构和功能组织的了解。生物学学生和其他研究人员将有充分的机会使用这些设备进行研究和培训