Electron Transfer Reactions Between Cytochrome c, Cytochrome c Oxidase and Dioxygen

细胞色素 c、细胞色素 c 氧化酶和双氧之间的电子转移反应

• 批准号: 0071777
• 负责人: Fred Hawkridge
• 金额: $ 49.27万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-15 至 2004-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0071777&HistoricalAwards=false
• 关键词: Electron; Transfer; Reactions; Between; Cytochrome

This project, which is supported in the Analytical and Surface Chemistry program develops new electrochemical methods for studying membrane-bound enzymes in complex media. Conducted by Dr. Albert Sneden and his group at Virginia Commonwealth University, the project prepares and characterizes stable assemblies of cytochrome c oxidase (CCO) adsorbed on a lipid bilayer that is anchored into a silver-coated gold electrode. A quartz crystal microbabance is used to coat the electrode with an optimum layer of silver. The electrode is then covered with octadectyl mercaptan to form an anchor for the bilayer. Atomic Force Microscopy is used to confirm the structure of the membrane. Electrochemical experiments have confirmed that such a bilayer mimics the in vivo reactivities of CCO. The known reaction between cyanide and CCO forms the basis for these studies. The electron transfer reaction between reduced cytochrome under flow conditions, CCO in the bilayer, and the electrode is characterized as a function of cyanide concentration, steady state turnover rate, and oxygen concentration. This research develops new electrochemical methods for studying membrane-bound enzymes in complex media. Results from this research will be useful for assessing the potential efficacy of compounds as enzyme inhibitors and will be important in areas such as drug testing.

该项目得到了分析和表面化学项目的支持，开发了新的电化学方法来研究复杂介质中的膜结合酶。 该项目由弗吉尼亚联邦大学的 Albert Sneden 博士和他的团队领导，准备并表征了吸附在固定在镀银金电极上的脂质双层上的细胞色素 C 氧化酶 (CCO) 的稳定组装体。使用石英晶体微球在电极上涂上一层最佳的银。然后用十八烷基硫醇覆盖电极以形成双层的锚。 原子力显微镜用于确认膜的结构。电化学实验已证实这种双层模拟了 CCO 的体内反应性。氰化物和 CCO 之间的已知反应构成了这些研究的基础。流动条件下还原细胞色素、双层中的 CCO 和电极之间的电子转移反应被表征为氰化物浓度、稳态周转率和氧浓度的函数。 这项研究开发了新的电化学方法来研究复杂介质中的膜结合酶。 这项研究的结果将有助于评估化合物作为酶抑制剂的潜在功效，并且在药物测试等领域非常重要。