Phylogeny of the Genera of Entomobryidae (Collembola)Based on Nuclear DNA Sequences and Morphology

基于核 DNA 序列和形态学的昆虫科（跳虫）属的系统发育

• 批准号: 0075548
• 负责人: Felipe Soto-Adames
• 金额: $ 23.5万
• 依托单位: University of Vermont & State Agricultural College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0075548&HistoricalAwards=false
• 关键词: Phylogeny; Genera; Entomobryidae; Collembola; Based

DEB-0075548Dr. Felipe Soto-AdamesDr. Felipe Soto-Adames at the University of Vermont has received a grant to study the evolution of a highly unusual group of insects known as springtails (Collembola, non-insect hexapods). Entomobryidae is the largest family of springtails accounting for 21% of all species described in the class. The family has a world wide distribution and in some tropical settings surpasses all other groups in number of species and individuals. Despite this diversity relatively little work has been done to elucidate their evolutionary relationships. This project is aimed at providing the first comprehensive estimate of phylogenetic relationships among genera of Entomobryidae using a data set that combines DNA sequences and morphology. The resulting phylogeny will accomplish three objectives: clarify relationships among the three largest groups of genera; test previous hypotheses suggesting a polyphyletic origin for Paronellidae; and provide amuch needed phylogenetic framework for future studies attempting to identify factors contributing to the successful diversification of this family. All genera of Entomobryidae will be examined to complete their morphological descriptions. Because morphological characters alone may not resolve some relationships, this data set will be complemented with DNA sequences from two nuclear genes. Elongation factor 1-alpha (EF1-a) and the small subunit of the ribosome (18S) are very conserved genes which allow rapid and consistent amplification via the polymerase chain reaction even for single individual springtails, some of which are 0.8mm long or less. Despite their high degree of sequence conservation analysis of preliminary data shows that in combination with morphology these genes can resolve relationships at the subfamily level. Resolution of the long standing problems of relationships among genera of Entomobryidae will result in a more stable classification, with more predictive power than the current system. In addition, Dr. Soto-Adames will update the key to genera of Entomobryidae already available in the internet. This key will be an excellent aid for agricultural entomologists given the recent increased use of Collembola as indicators of soil health. Soil biologists will also benefit from clarifications of the taxonomy

DEB-0075548博士菲利普·索托-亚当斯博士佛蒙特大学的费利佩·索托-亚当斯获得了一笔赠款，用于研究一种非常不寻常的昆虫群体的进化，这种昆虫被称为跳虫（弹尾目，非昆虫六足动物）。 虫蛉科是跳虫中最大的一科，占所有种类的21%。 该科分布于世界各地，在某些热带环境中，其物种和个体数量超过了所有其他类群。 尽管这种多样性相对较少的工作已经做了阐明他们的进化关系。 该项目的目的是提供第一个全面的估计虫螨科属之间的系统发育关系使用的数据集，结合DNA序列和形态。由此产生的系统发育将完成三个目标：澄清三个最大的属群之间的关系;测试以前的假设，表明一个多系起源Paronellidae;并提供amuch所需的系统发育框架，为未来的研究，试图确定因素有助于成功的多样化，这个家庭。 所有属的虫蛉科将进行检查，以完成其形态描述。 由于形态特征本身可能无法解决某些关系，因此将用来自两个核基因的DNA序列补充该数据集。 延伸因子1-α（EF 1-a）和核糖体的小亚基（18 S）是非常保守的基因，其允许通过聚合酶链反应快速和一致的扩增，即使对于单个跳虫，尽管它们的序列高度保守，但对初步数据的分析表明，结合形态学，这些基因可以解决子家族水平。 解决虫虱科属间关系的长期问题将导致一个更稳定的分类，具有比当前系统更强的预测能力。 此外，Soto-Adames博士将更新互联网上已有的虫螨科属的检索表。 鉴于最近弹尾虫作为土壤健康指标的使用越来越多，这一关键将是农业昆虫学家的一个很好的帮助。 土壤生物学家也将受益于分类学的澄清