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The Genetic and Molecular Basis of Sex Determination in Ceratopteris

角蕨性别决定的遗传和分子基础

基本信息

批准号：
0077558
负责人：
Jo Ann Banks
金额：
$ 45万
依托单位：
Purdue Research Foundation
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-01 至 2004-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0077558&HistoricalAwards=false
关键词：
Genetic Molecular Basis Sex Determination

项目摘要

AbstractGametophytes of the fern Ceratopteris richardii are sexually dimorphic, either hermaphroditic or male. The determinant of sex in this and other ferns is antheridiogen, a gibberellin-like pheromone that is secreted by the hermaphroditic gametophyte and promotes male development of other sexually undetermined gametophytes. The plant hormone abscisic acid blocks the antheridiogen response. The primary objective of this proposal is to understand how antheridiogen and abscisic acid govern the sex of the Ceratopteris gametophyte. The genetic analysis of five phenotypic classes of sex-determining mutants (12 loci) has led to a hypothetical sex-determining genetic pathway in this species. To understand how these genes regulate sex determination at the molecular level, it will be necessary to clone these genes. The objectives of the proposed research are to continue the genetic analysis of sex determination and develop methods required to clone the sex-determining genes in Ceratopteris. These methods include transformation, which will allow us to incorporate reverse genetic approaches to study gene function, and endogenous transposable element identification, which will provide new options to clone the many genes that have been identified by mutation. Understanding how sex is determined in plants is an important developmental question whose answer may ultimately have practical applications in agriculture and plant breeding. The proposed studies will provide a basic understanding of the genes involved in this process and will also be of value in understanding how the fate of a plant cell (the fern spore) is ultimately regulated by antheridiogen, a gibberellin-like pheromone.

摘要水蕨配子体具有两性和雄性两种类型。这种蕨类植物和其他蕨类植物的性别决定因素是雄激素，一种类似赤霉素的信息素，由两性配子体分泌，促进其他性别未定的配子体的雄性发育。植物激素脱落酸阻断雄激素反应。本研究的主要目的是了解促精子素和脱落酸如何调控水蕨属配子体的性别。对5个性别决定突变体表型类型（12个基因座）的遗传分析表明，该物种可能存在一条性别决定的遗传途径。为了了解这些基因如何在分子水平上调节性别决定，有必要克隆这些基因。拟议研究的目标是继续进行性别决定的遗传分析，并开发克隆水蕨性别决定基因所需的方法。这些方法包括转化，这将使我们能够将反向遗传学的方法来研究基因的功能，和内源性转座因子的鉴定，这将提供新的选择，克隆许多基因已被确定的突变。了解植物的性别是如何决定的是一个重要的发展问题，其答案最终可能在农业和植物育种中有实际应用。拟议中的研究将提供一个基本的了解基因参与这一过程，也将是有价值的了解植物细胞（蕨类孢子）的命运是如何最终调控的antheridiogen，赤霉素样信息素。