Acquisition of an 800MHz Nuclear Magnetic Resonance Spectrometer for Structural Biology

购买用于结构生物学的 800MHz 核磁共振波谱仪

• 批准号: 0079604
• 负责人: Peter Gettins
• 金额: $ 150.5万
• 依托单位: University of Illinois at Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2004-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0079604&HistoricalAwards=false
• 关键词: Acquisition; 800MHz; Nuclear; Magnetic; Resonance

The research to be conducted on the new instrument funded by this award relates to solution structural studies on biomolecules using nuclear magnetic resonance (NMR) spectroscopy. It will be directed at aspects of protein structure and dynamics in solution and will involve determination of structures of medium to large proteins, both alone and in complex with small peptides, DNA oligonucleotides or other proteins, and will include examination of dynamics of regions important for recognition of other proteins or DNA. These studies are of a basic nature and will provide mechanistic understanding of processes of ligand recognition and mode of action. Four principal research groups at the University of Illinois at Chicago and an investigator in the chemistry department at nearby Loyola University of Chicago will be the major users of the instrument. Projects include (i) understanding the specificity of binding of the multiple protein ligands of the broad specificity clearance receptor LRP that is involved in metabolism of proteases and lipoproteins, (ii) understanding the molecular basis for recognition of regulatory DNA sequences by a transcription factor protein family, (iii) determination of the structure of domains from the erythrocyte skeletal protein spectrin that are responsible for the shape and flexibility of red blood cells (iv) examination of the mechanism of infection of cells by HIV. The award will be used to purchase a state-of-the art narrow bore 800MHz NMR spectrometer fully equipped for multinuclear, multidimensional, gradient enhanced solution structural studies on bio-macromolecules. The instrument will be housed in custom-designed space. This instrument will complement an existing 600MHz NMR spectrometer that is currently available on campus for determination of protein solution structures, but that lacks the capabilities of the higher field instrument for tackling the very challenging problems described. The 800MHz NMR spectrometer and the studies to be carried out on it will be of great benefit both for improving the research infrastructure of the university and of the Chicago structural biology community and for enhancing the training opportunities for the area students. State-of-the-art highest field NMR instrumentation, as represented by the awarded equipment, will be unique in metropolitan Chicago and will permit the continued development of already active NMR programs into the more challenging, but also most exciting and rewarding areas. This will contribute significantly to the competitiveness of these research programs, their success and hence visibility, which in turn will help both in recruitment of additional structurally-oriented faculty, postdocs and students and in fostering collaborations between faculty within the university and Chicago. All of the investigators currently train both graduate students and postdocs and so offer training opportunities in use of NMR to examine protein structure; an area that is already of the most fundamental importance to all biological sciences, and that will continue to grow in the post-genomic era. The acquisition of an 800MHz spectrometer will enable such students to work and train on the most challenging projects and so acquire skills that will be needed for careers in the critical biotechnology, pharmaceutical, and molecular medicine industries.

该奖项资助的新仪器上进行的研究涉及使用核磁共振（NMR）光谱对生物分子进行溶液结构研究。 它将针对蛋白质的结构和动力学方面的解决方案，并将涉及中大型蛋白质的结构的测定，无论是单独的和与小肽，DNA寡核苷酸或其他蛋白质的复合物，并将包括对其他蛋白质或DNA的识别重要的区域的动力学检查。 这些研究是一个基本的性质，并将提供配体识别和作用模式的过程的机械理解。 位于芝加哥的伊利诺伊大学的四个主要研究小组和附近的芝加哥洛约拉大学化学系的一名研究人员将是该仪器的主要用户。 项目包括（i）了解参与蛋白酶和脂蛋白代谢的广泛特异性清除受体LRP的多种蛋白配体结合的特异性，（ii）了解转录因子蛋白家族识别调控DNA序列的分子基础，（iii）确定来自红细胞骨架蛋白血影蛋白的结构域的结构，所述结构域负责红细胞的形状和柔性（iv）研究HIV感染细胞的机制。 该奖项将用于购买最先进的窄孔800 MHz NMR光谱仪，该光谱仪配备齐全，可用于生物大分子的多核，多维，梯度增强溶液结构研究。 该仪器将放置在定制设计的空间中。 该仪器将补充现有的600 MHz NMR光谱仪，该光谱仪目前可在校园内用于确定蛋白质溶液结构，但缺乏更高场仪器的能力来解决所描述的非常具有挑战性的问题。 800 MHz核磁共振波谱仪和对其进行的研究将极大地有利于改善大学和芝加哥结构生物学社区的研究基础设施，并提高该地区学生的培训机会。 以获奖设备为代表的最先进的最高场核磁共振仪器在大都市芝加哥将是独一无二的，并将使已经活跃的核磁共振项目继续发展到更具挑战性，但也是最令人兴奋和最有价值的领域。 这将大大有助于这些研究项目的竞争力，他们的成功，因此知名度，这反过来又将有助于招聘更多的结构导向的教师，博士后和学生，并在大学和芝加哥内促进教师之间的合作。 所有的研究人员目前都在培训研究生和博士后，因此提供了使用NMR检查蛋白质结构的培训机会;这一领域已经对所有生物科学具有最根本的重要性，并将在后基因组时代继续发展。 800 MHz光谱仪的收购将使这些学生能够在最具挑战性的项目上工作和培训，从而获得关键生物技术，制药和分子医学行业职业所需的技能。