PROPOSAL FOR ACQUISITION OF AN 800MHZ NMR SPECTROMETER

本提案请求支持购买一台800 MHz核磁共振光谱仪，供加州大学伯克利分校的研究人员用于生物分子结构、功能和动力学的研究。主要用户将是Wemmer、Tinoco和Handel教授小组，他们已经非常积极地将核磁共振光谱应用于生物问题。正在解决的问题包括NtrC的结构，细菌双组分信号系统，序列特异性DNA结合配体的设计，I族内含子RNA和溴花叶病毒RNA 3'端的元件结构，趋化因子及其相互作用伙伴的结构，以及具有重新包装核心的蛋白质。还有一大批二级用户，他们的实验室集中于特定的生物学问题，但其系统已发展到适合进行结构研究并将促进进一步的生化实验的程度。这些用户和他们的研究领域包括Marqusee教授（蛋白质折叠的自由能景观），Nogales教授（微管蛋白相互作用和组成转录因子IID的复合体），Bustamante教授（I组内含子机械展开及其与结构特征的关系），Collins教授（端粒酶成分结构），Berger教授（Cdc6和DnaG结构域的结构）和Botchan教授（E1和E2结构域的结构和相互作用）。

This proposal requests support toward the purchase of an 800 MHz NMR spectrometer that will be used by investigators at the University of California at Berkeley for studies of biomolecular structure, function and dynamics. The principle users will be the groups of Professors Wemmer, Tinoco and Handel, who are already very active in applications of NMR spectroscopy to biological problems. Problems being addressed include the structure of NtrC, a bacterial two-component signaling system, design of sequence specific DNA binding ligands, structures of elements of group I intron RNA and brome mosaic virus RNA 3' end, structures of chemokines and their interacting partners, and proteins with repacked cores. There is also a substantial group of secondary users whose labs are focused on particular biological problems, but have systems which are developed to the point that structural studies are appropriate and would facilitate further biochemical experiments. These users and their areas of research include Professors Marqusee (free energy landscapes for protein folding), Nogales (tubulin interactions, and complexes making up transcription factor IID), Bustamante (group I intron mechanical unfolding and the relationship to structural features), Collins (structures of telomerase components), Berger (structure of Cdc6 and DnaG domains) and Botchan (structure and interactions of E1 and E2 domains).