Integration of Fluorescence Microscopy and Imaging Technology Into a Liberal Arts Biology Curriculum

生物科学(61)这个综合性项目正在将新的显微镜和成像技术整合到本科生物学课程中。除了将相差显微镜纳入我们的第一年课程外，我们还利用单激发波长荧光、双波长荧光比率成像和其他类型的显微镜技术来研究细胞的生态、生理和细胞过程。我们正在为我们的入门生物学实验室系列以及细菌学和细胞生理学以及系内其他高级实验室开发新的实验。我们相信，改进我们的课程，专注于细胞，将为学生理解分子如何与细胞并最终与生物体联系起来建立必要的联系。目前对细胞及其功能的了解的一个主要组成部分是使用荧光显微镜和成像分析。该设备被用于各种课程，从入门实验室开始，我们有许多潜在的生物专业、护理专业、体育专业以及环境科学和分子生物学/生物化学附属专业的学生。我们还在微生物学、细菌学和细胞生理学中使用显微镜。这些设备还将用于为生物学专业和环境科学宣传材料开发的环境微生物学课程，以及为非专业学生开发的名为极端生活的新课程。这项工作的动机是美国微生物学会的建议，建议的方法是从科学文献中挑选出来的。

Biological Sciences (61) This comprehensive project is integrating new microscopic and imaging technology into the undergraduate biology curriculum. In addition to the incorporation of phase contrast microscopy into our first year curriculum, we are incorporating experiments using techniques such as single excitation wavelength epifluorescence, dual wavelength fluorescent ratio imaging, and other types of microscopy in studying the ecology, physiology, and cellular processes of cells. We are developing new experiments for our Introductory Biology lab series as well as in Bacteriology and Cell Physiology and other upper-level labs in the department. We believe that improving our courses focusing on cells will make a needed connection for the students in understanding how the molecules relate back to the cells and eventually to the organism. A major component in current understandings of cells and how they function is the use of epifluorescence microscopy and imaging analysis. This equipment is being used in a variety of courses starting with the introductory labs where we have many prospective biology majors, nursing majors, physical education majors as well as students involved in both the environmental science and molecular biology/ biochemistry collateral majors. We are also using the microscopes in the Microbiology, Bacteriology, and Cell Physiology. The equipment will also be used in an Environmental Microbiology course being developed for Biology majors and the Environmental Science collateral and in a new course for non-majors called Life at the Extremes. The effort is motivated by recommendations of the American Society of Microbiology from which suggested approaches are adapted, as well as techniques culled from the scientific literature.