Monitor single-cell dynamics using optically computed phase microscopy in correlation with fluorescence characterization of intracellular properties
使用光学计算相位显微镜监测单细胞动力学与细胞内特性的荧光表征相关
基本信息
- 批准号:10589414
- 负责人:
- 金额:$ 44.59万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-01-01 至 2025-12-31
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalAddressAdhesionsAdhesivesAffectArchitectureArthritisAtherosclerosisBindingBiomedical ResearchCell AdhesionCell SurvivalCellsCharacteristicsClinical ResearchCollaborationsComplexContrast MediaDataData AnalysesDetectionDevelopmentDiseaseDoxorubicinEarly DiagnosisExposure toExtracellular MatrixFluorescenceFluorescence MicroscopyFunctional disorderFundingGlutathione DisulfideHela CellsImageImaging TechniquesImaging technologyInterdisciplinary StudyInterferometryInvestigationKnowledgeLabelLateralLeadLightMalignant NeoplasmsMeasurementMeasuresMechanicsMethodsMicroscopicMicroscopyMitochondriaMonitorMotionMultimodal ImagingOpticsOrganellesOsteoporosisPatientsPharmaceutical PreparationsPhasePlayPositioning AttributeProcessProliferatingPropertyProtocols documentationResearchResearch AssistantResearch PersonnelResearch Project GrantsResolutionRoleSemanticsSignal TransductionStructureTimeTrainingTrypsinValidationbiomaterial developmentcareercell behaviorcell preparationcellular imagingchemotherapydata acquisitiondeep neural networkexperimental studyfundamental researchhigh throughput technologyimage archival systemimage processingimaging capabilitiesimaging platformmicroscopic imagingmigrationnanoscalenext generationoptical imagingphoton-counting detectorpreclinical studyquantitative imagingtechnological innovationtechnology developmentundergraduate researchundergraduate student
项目摘要
Project Summary
The objective of this study is to develop a multi-modality imaging platform that integrates complex phase
microscopy (CPM) based on optical computation and fluorescence microscopy (FM). We will use the imaging platform to
correlate the sub-cellular dynamic motion and intracellular properties to study cell adhesion which plays an important role
in many aspects of cell behavior. This study is significant for fundamental research, preclinical and clinical study, and
technology development. By simultaneously imaging cell motion and organelle properties, CPM-FM enables the
investigation of cell dynamics in correlation biomolecular characteristics. CPM-FM investigation of cell adhesion will result
in better understanding of pathophysiology of different diseases, open the door to new methods for disease treatment and
early diagnosis, lead to the development biomaterials that need specific interaction with cells, and benefit patients and
clinicians. This study will also establish the feasibility of CPM as an imaging technology that allows non-invasive, and
continuous monitoring of cell activities without contrast agents. We will achieve the objective of this study through
developing the CPM imaging platform (Aim 1), investigating high-throughput single-cell dynamic imaging using CPM
(Aim 2) and investigating the correlation between adhesive properties using CPM and cell viability using fluorescence
microscopy (Aim 3). This project will provide a unique platform for undergraduate students to conduct research.
Undergraduate research assistants will play major roles in cell imaging, image processing, and data analysis. Undergraduate
research assistants participating in this study are exposed to multi-disciplinary research that will prepare them for a career
in the fields of biomedicine.
项目摘要
本研究的目的是开发一种多模态成像平台,
基于光学计算和荧光显微术(FM)的CPM显微术。我们将使用成像平台,
将亚细胞的动态运动与细胞内的特性联系起来,研究细胞间的粘附,
在细胞行为的许多方面。本研究对基础研究、临床前和临床研究具有重要意义,
技术开发。通过同时成像细胞运动和细胞器特性,CPM-FM使细胞运动和细胞器特性成为可能。
研究细胞动力学与生物分子特性相关性。细胞粘附的CPM-FM研究将导致
更好地了解不同疾病的病理生理学,为疾病治疗的新方法打开大门,
早期诊断,导致开发需要与细胞特异性相互作用的生物材料,并使患者受益,
临床医生本研究还将确定CPM作为一种非侵入性成像技术的可行性,
在没有造影剂的情况下连续监测细胞活性。我们会透过以下方法达致这项研究的目的:
开发CPM成像平台(目标1),研究使用CPM的高通量单细胞动态成像
(Aim 2)使用CPM研究粘附特性与使用荧光研究细胞活力之间的相关性
显微镜(目的3)。该项目将为本科生提供一个独特的平台进行研究。
本科研究助理将在细胞成像,图像处理和数据分析中发挥重要作用。本科
参与这项研究的研究助理将接触多学科研究,为他们的职业生涯做好准备
在生物医学领域。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Xuan Liu其他文献
Xuan Liu的其他文献
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{{ truncateString('Xuan Liu', 18)}}的其他基金
Multi-modality optical imaging of single-cell dynamics using supercontinuum light source
使用超连续谱光源的单细胞动力学多模态光学成像
- 批准号:
10798646 - 财政年份:2023
- 资助金额:
$ 44.59万 - 项目类别:
Optically computed compressive OCT for ultra-high speed phase-resolved dynamic imaging
用于超高速相位分辨动态成像的光学计算压缩 OCT
- 批准号:
10116602 - 财政年份:2020
- 资助金额:
$ 44.59万 - 项目类别:
Optically computed compressive OCT for ultra-high speed phase-resolved dynamic imaging
用于超高速相位分辨动态成像的光学计算压缩 OCT
- 批准号:
10321947 - 财政年份:2020
- 资助金额:
$ 44.59万 - 项目类别:
ALCOHOL MODULATES HIV-1 REPLICATION IN LATENT CD4+ CELLS
酒精调节潜伏 CD4 细胞中的 HIV-1 复制
- 批准号:
6611469 - 财政年份:2002
- 资助金额:
$ 44.59万 - 项目类别:
ALCOHOL MODULATES HIV-1 REPLICATION IN LATENT CD4+ CELLS
酒精调节潜伏 CD4 细胞中的 HIV-1 复制
- 批准号:
6796186 - 财政年份:2001
- 资助金额:
$ 44.59万 - 项目类别:
ALCOHOL MEDIATED HIV-1 INFECTIVITY AND REPLICATION
酒精介导的 HIV-1 感染和复制
- 批准号:
6654948 - 财政年份:2001
- 资助金额:
$ 44.59万 - 项目类别:
ALCOHOL MEDIATED HIV-1 INFECTIVITY AND REPLICATION
酒精介导的 HIV-1 感染和复制
- 批准号:
6322482 - 财政年份:2001
- 资助金额:
$ 44.59万 - 项目类别:
ALCOHOL MODULATES HIV-1 REPLICATION IN LATENT CD4+ CELLS
酒精调节潜伏 CD4 细胞中的 HIV-1 复制
- 批准号:
6533715 - 财政年份:2001
- 资助金额:
$ 44.59万 - 项目类别:
ALCOHOL MODULATES HIV-1 REPLICATION IN LATENT CD4+ CELLS
酒精调节潜伏 CD4 细胞中的 HIV-1 复制
- 批准号:
6454373 - 财政年份:2001
- 资助金额:
$ 44.59万 - 项目类别:
ALCOHOL MEDIATED HIV-1 INFECTIVITY AND REPLICATION
酒精介导的 HIV-1 感染和复制
- 批准号:
6533658 - 财政年份:2001
- 资助金额:
$ 44.59万 - 项目类别:
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