Analysis of Exit From Stationary Phase In Yeast: Gene Expression and Its Consequences.

0092374玛格丽特·沃纳-沃什伯恩这项研究项目将研究细胞退出静止期时的基因表达。基因芯片分析将确定信使核糖核酸的积累动态，包括信使核糖核酸的周转率。将使用集群和可视化程序VxInsight进行数据分析，以确定具体的监管模式。选定的转录本的相对丰度将通过Northern分析得到证实。为了确定在这个过程中诱导的基因是否是退出稳定期所必需的，携带在稳定期高度诱导和再增殖的基因缺失的菌株将被分析生长期特定缺陷和其他与应激相关的表型。这将通过微阵列和更经典的对携带基因缺失的菌株的生长分析来完成，这些基因的mRNAs在这些过程中最丰富。这项工作将有助于我们在基因组水平上理解微生物生理学，并为静止细胞的动力学提供洞察力。

0092374 Margaret Werner-WashburneThis research project will examine gene expression as cells exit from stationary phase. The dynamics of mRNA accumulation, including rates of mRNA turnover, will be determined using microarray analysis. Data analysis will be carried out using the clustering and visualization program, VxInsight, to identify specific patterns of regulation. The relative abundance of selected transcripts will be confirmed by northern analysis. To determine if genes induced during this process are essential for exit from stationary phase, strains carrying deletions of genes that are highly induced during stationary phase and reproliferation will be analyzed for growth-phase specific defects and other stress-related phenotypes. This will be done by both microarray and more classical growth analyses of strains carrying deletions in genes whose mRNAs are most abundant during these processes. This work will contribute to our understanding of microbial physiology at the genomic level and provide insights into the dynamics of quiescent cells.