Analysis of Exit From Stationary Phase In Yeast: Gene Expression and Its Consequences.

酵母退出静止期的分析：基因表达及其后果。

• 批准号: 0092374
• 负责人: Margaret Werner-Washburne
• 金额: $ 39万
• 依托单位: University of New Mexico
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0092374&HistoricalAwards=false
• 关键词: Analysis; Exit; Stationary; Phase; Yeast

0092374 Margaret Werner-WashburneThis research project will examine gene expression as cells exit from stationary phase. The dynamics of mRNA accumulation, including rates of mRNA turnover, will be determined using microarray analysis. Data analysis will be carried out using the clustering and visualization program, VxInsight, to identify specific patterns of regulation. The relative abundance of selected transcripts will be confirmed by northern analysis. To determine if genes induced during this process are essential for exit from stationary phase, strains carrying deletions of genes that are highly induced during stationary phase and reproliferation will be analyzed for growth-phase specific defects and other stress-related phenotypes. This will be done by both microarray and more classical growth analyses of strains carrying deletions in genes whose mRNAs are most abundant during these processes. This work will contribute to our understanding of microbial physiology at the genomic level and provide insights into the dynamics of quiescent cells.

[09:23 . 74] Margaret werner - washburn这个研究项目将检测细胞从固定期退出时的基因表达。mRNA积累的动态，包括mRNA周转率，将使用微阵列分析确定。数据分析将使用聚类和可视化程序VxInsight进行，以确定具体的监管模式。所选转录本的相对丰度将通过northern分析来确定。为了确定在这一过程中诱导的基因是否对退出静止期至关重要，将对携带在静止期和再生期高度诱导的基因缺失的菌株进行生长期特异性缺陷和其他应激相关表型的分析。这将通过微阵列和更经典的菌株生长分析来完成，这些菌株携带在这些过程中mrna最丰富的基因缺失。这项工作将有助于我们在基因组水平上对微生物生理学的理解，并为静止细胞的动力学提供见解。