SGER: Separation of Polyethylene Wear Debris Found in Synovial Fluid Generated from Total Joint Prostheses Using Bio-Ferrography

SGER：使用生物铁谱技术分离全关节假体产生的滑液中发现的聚乙烯磨损碎片

• 批准号: 0092944
• 负责人: Donna Meyer
• 金额: $ 5.48万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2002-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0092944&HistoricalAwards=false
• 关键词: SGER; Separation; Polyethylene; Wear; Debris

0092944MeyerThe orthopedic community would benefit from knowing the ratio of the various constituents contained in the synovial fluid of patients with total hip and knee replacements before the onset of complications leading to revision surgery. Aspirating the joints of patients with indications is recommended most specifically prior to revision surgery. It is the aspirate from these patients that will be used as the basis for the proposed set of experiments. The ratios of the constituents to each other may be used by physicians as one of several tools in the decision whether to perform revision surgery. There may also be instances in which surgery will not be recommended based in part on the particulate ratios, in which case benefits to both the patient and the economy may be realized. The clinical relevance would be that the surgeon now has a quasi-inside look at the prosthesis before surgery, offering some information about what area(s) of the implant may require revision. This is not information that can be readily seen in x-rays or by other visual means. An SGER award supports research to examine the feasibility of using a technique called Bio-Ferrography to separate the various types of wear particles which form in total hip and knee replacements as they wear. The PI has access to a unique collection of over one hundred well-documented samples of synovial fluid aspirate which has been obtained from total hip and knee replacements prior to revision surgery. The primary wear particles are ultra high molecular weight polyethylene (UHMWPE) from the acetabular cup, metal particles such as stainless steel or cobalt-cromium-molybdenum from the femoral head, particles of bone cement used for fixation of the prostheses at the bone/implant interface, and bone particulate itself. The particles will be tagged with magnetic tagging molecules which make it possible to sort them by the magnetic field in the bio-ferrograph. Identification of suitable tagging molecules, sorting of the particles by material and size, and relating the nature and amount of wear particles to observations made of the worn joint during and after surgery will be the main components of the study.***

0092944Meyer 在出现导致修正手术的并发症之前，了解全髋关节和膝关节置换术患者滑液中所含各种成分的比例将使骨科界受益。特别建议在修复手术之前对有指征的患者进行关节抽吸。这些患者的抽吸物将用作拟议的一组实验的基础。医生可以使用各成分之间的比例作为决定是否进行修复手术的几种工具之一。也可能存在部分基于颗粒比率而不会推荐手术的情况，在这种情况下，可以实现对患者和经济的益处。 临床相关性在于，外科医生现在可以在手术前对假体进行准内部观察，提供有关植入物的哪些区域可能需要修正的一些信息。这不是 X 射线或其他视觉手段可以轻易看到的信息。 SGER 奖项支持研究使用生物铁谱技术来分离全髋关节和膝关节置换术中磨损时形成的各种类型的磨损颗粒的可行性。 PI 可以获得超过 100 个有详细记录的滑液抽吸样本的独特集合，这些样本是在翻修手术之前从全髋关节和膝关节置换术中获得的。主要磨损颗粒是髋臼杯中的超高分子量聚乙烯 (UHMWPE)、股骨头中的不锈钢或钴铬钼等金属颗粒、用于将假体固定在骨/植入物界面处的骨水泥颗粒以及骨颗粒本身。 这些颗粒将被标记上磁性标记分子，从而可以通过生物铁谱仪中的磁场对它们进行分类。 识别合适的标记分子，按材料和尺寸对颗粒进行分类，并将磨损颗粒的性质和数量与手术期间和术后磨损关节的观察结果联系起来。***