Role of Piriformospora indica in sulphur metabolism in Arabidopsis thaliana

印度梨形孢在拟南芥硫代谢中的作用

• 批准号: 146371860
• 负责人: Professor Dr. Ralf Oelmüller
• 金额: --
• 依托单位: Lehrstuhl für Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/146371860?language=en
• 关键词: Role; Piriformospora; indica; sulphur; metabolism

Pirirformospra indica is an endophytic fungus which colonizes the roots of many plant species and promotes their growth, seed production and resistance against biotic and abiotic stress. An Arabidopsis mutant impaired in many responses to the fungus has a lesion in SLIM1, a central transcriptional regulator of sulphur (S) metabolism, glucosinolate biosynthesis and a component of the induced systemic resistance (ISR) against pathogens. Another mutant is defective in MYB28, a regulator of the aliphatic glucosinolate biosynthesis in Arabidopsis. This demonstrates that transcription factors controling S and glucosinolate metabolism are specific targets of P. indica. The aim of the proposal is to understand how the fungus controls (a) the basic S metabolism, (b) the glucosinolate biosynthesis and (c) the ISR through SLIM1. We will test the hypothesis that P. indica controls the S metabolism in Arabidopsis for two reasons: to ensure that S-containing components are not limiting for plant growth and development, in particular under S limitation, and that sufficient Scontaining defense components prevent overcolonization of the roots, which would result in a shift from mutualism to parasitism. Furthermore, we will analyse how P. indica confers ISR by controlling SLIM1 and its interaction partner MYB72.

菠萝蜜是一种内生真菌，它寄生在多种植物的根部，促进植物的生长、种子生产和对生物和非生物胁迫的抗性。拟南芥突变体对该真菌的许多反应受损，在SLIM1中有损伤，SLIM1是硫代谢的中心转录调节因子(S)，硫代葡萄糖苷的生物合成以及诱导的对病原菌的系统抗性(ISR)的一个组成部分。另一个突变体在MYB28中存在缺陷，MYB28是拟南芥中脂肪硫代葡萄糖苷生物合成的调节因子。这说明控制S代谢和硫代葡萄糖苷代谢的转录因子是稻纵卷叶蛾的特异靶标。该提案的目的是了解该真菌如何通过SLIM1控制(A)S的基本新陈代谢，(B)硫代葡萄糖苷的生物合成和(C)ISR。我们将验证这一假说：一是确保含有S的成分不会限制植物的生长和发育，特别是在S限制下；二是要确保足够的含硫防御成分防止根部过度定殖，从而导致从互惠关系向寄生关系的转变。此外，我们还将分析稻瘟病菌是如何通过控制SLIM1及其互作伙伴MYB72来赋予ISR的。