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Barley compatibility factors pivotal for root colonisation and manipulation of basal defence by Piriformospora indica

大麦相容性因素对于印度梨形孢菌根部定植和基础防御的操纵至关重要

基本信息

批准号：
19984878
负责人：
Professor Dr. Karl-Heinz Kogel
金额：
--
依托单位：
Abteilung Phytopathologie
依托单位国家：
德国
项目类别：
Research Units
财政年份：
2006
资助国家：
德国
起止时间：
2005-12-31 至 2012-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/19984878?language=en
关键词：
Barley compatibility factors pivotal root

项目摘要

This project is aimed at the characterization of the systemic reprogramming in barley, which modulates the compatible interaction with the biotrophic leaf pathogen Blumeria graminis f.sp. hordei upon root infestation with the mutualistic endophyte Piriformospora indica. We have recently shown that the basidiomycete P. indica - upon successful establishment in the roots - reprograms barley to salt stress tolerance, resistance to root diseases and higher yield (Waller et al., 2005). Successful powdery mildew infections in barley leaves are also disturbed by the mutualistic fungus. These processes are associated with a strong change in plant metabolism, especially with a drastic alteration of leaf and root antioxidants. On the basis of these findings we will perform an in-depth analysis of the barley metabolome (B6) and transcriptome (B7) with two specific foci: First, to elucidate the process of establishment of the mutualistic fungus within the barley roots; second, to characterize elements of the systemic response in leaves leading to an interruption or failure of compatibility processes required for successful establishment of biotrophic leaf pathogens like Blumeria. New gene candidates will be pre-selected systematically for their regulatory role in compatibility by means of transiently transformed barley leaves upon Blumeria inoculation. Stable transgenic barley and maize lines (B3) generated with verified gene candidates and genes identified by other projects (A1, A2, B5, B6) will be tested with Blumeria and P. indica. By comparing candidate genes in the different plant - microbe systems, we will identify common regulatory processes, metabolites and metabolic networks implicated in compatibility including those required for successful interactions with mutualistic fungi.

该项目旨在表征大麦中的系统重编程，其调节与共生内生菌Piriformospora indica根侵染后与活体营养叶病原体大麦白粉病菌的相容性相互作用。我们最近已经表明，担子菌P. indica -在根中成功建立-将大麦重编程为盐胁迫耐受性、对根病的抗性和更高的产量（Waller等人，2005年）。大麦叶片中成功的白粉病感染也受到互利共生真菌的干扰。这些过程与植物代谢的强烈变化有关，特别是叶和根抗氧化剂的急剧变化。在此基础上，我们将对大麦的代谢组（B6）和转录组（B7）进行深入分析，其中有两个特定的焦点：第一，阐明互惠真菌在大麦根内的建立过程;第二，表征导致成功建立活体营养型叶病原体所需的相容性过程中断或失败的叶中系统反应的要素就像布氏花新的候选基因将预先选择系统的兼容性，通过瞬时转化大麦叶片接种后的调节作用。将用Blumeria和P. indica测试用经验证的候选基因和由其他项目（A1、A2、B5、B6）鉴定的基因产生的稳定的转基因大麦和玉米品系（B3）。通过比较不同植物-微生物系统中的候选基因，我们将确定与相容性有关的共同调控过程、代谢物和代谢网络，包括与互利共生真菌成功相互作用所需的那些。