Protein Crystals: New Templates for the Construction of Nanostructured Solids

蛋白质晶体：构建纳米结构固体的新模板

• 批准号: 0103174
• 负责人: Vicki Colvin
• 金额: $ 120万
• 依托单位: William Marsh Rice University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0103174&HistoricalAwards=false
• 关键词: Protein; Crystals; New; Templates; Construction

Divisions of Chemistry, Materials Research, Bioengineering and Environmental Systems, and Molecular and Cellular Biosciences support this multidivisional award to William Marsh Rice University. This Nanoscale Interdisciplinary Research Team (NIRT) proposal was submitted in response to the solicitation "Nanoscale Science and Engineering" (NSF 00-119). Under this project, Vicki Colvin, a material chemist with experience in template chemistry, Daniel Mittleman, an optical engineer and George Phillips, a biologist with expertise in protein crystal growth, will develop crystalline and monolithic nanostructured metal and ceramic materials using crystals of streptavidin, lysozyme and related proteins. These protein crystalline structures will be reinforced by glutaraldehyde cross-linking before the templating. X-ray diffraction studies and atomic force microscope imaging will be used to characterize the prepared materials with respect to crystal symmetry, periodicity and overall quality of the crystal arrangement. These porous and three-dimensional open crystalline inorganic structures with nanometer spacing may find applications in diffraction gradients, lenses, mirrors and other devices for soft X-ray optics. The research program will also provide a rich multidisciplinary education and training opportunities in material and protein chemistries to postdoctoral, graduate and undergraduate students.Under the award, ordered mesoporous nanostructured metal and ceramic materials will be fabricated using cross-linked protein crystals as sacrificial templates. The broader impact of the project is the understanding the formation of nanostructures, the templating effect of different protein crystal scaffolding, and the determination of optical properties of the mesoporous structures prepared in the soft X-ray range of the spectrum. In addition, the research program will provide a rich multidisciplinary education and training opportunities in materials chemistry, protein chemistry and optics to graduate and undergraduate students.

化学、材料研究、生物工程和环境系统以及分子和细胞生物科学部门支持威廉·马什·莱斯大学获得这一多部门奖项。该纳米跨学科研究团队 (NIRT) 提案是为了响应“纳米科学与工程”(NSF 00-119) 征集而提交的。 在该项目下，具有模板化学经验的材料化学家 Vicki Colvin、光学工程师 Daniel Mittleman 和具有蛋白质晶体生长专业知识的生物学家 George Phillips 将利用链霉亲和素、溶菌酶和相关蛋白质的晶体开发晶体和整体纳米结构金属和陶瓷材料。 这些蛋白质晶体结构将在模板化之前通过戊二醛交联得到增强。 X射线衍射研究和原子力显微镜成像将用于表征所制备材料的晶体对称性、周期性和晶体排列的整体质量。这些具有纳米间距的多孔三维开放晶体无机结构可应用于衍射梯度、透镜、镜子和其他软 X 射线光学器件。 该研究项目还将为博士后、研究生和本科生提供材料和蛋白质化学方面丰富的多学科教育和培训机会。根据该奖项，将使用交联蛋白质晶体作为牺牲模板来制造有序介孔纳米结构金属和陶瓷材料。该项目更广泛的影响是了解纳米结构的形成、不同蛋白质晶体支架的模板效应，以及确定在软 X 射线光谱范围内制备的介孔结构的光学性质。 此外，该研究项目还将为研究生和本科生提供材料化学、蛋白质化学和光学方面丰富的多学科教育和培训机会。