SBIR Phase I: Cluster Ion Matrix Assisted Laser Desorption Ionization (MALDI) - MS/MS by Coincidence

SBIR 第一阶段：簇离子基质辅助激光解吸电离 (MALDI) - 巧合 MS/MS

• 批准号: 0109700
• 负责人: J. Albert Schultz
• 金额: $ 9.96万
• 依托单位: IONWERKS, INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0109700&HistoricalAwards=false
• 关键词: SBIR; Phase; Cluster; Ion; Matrix

This Small Business Innovation Research Phase I (SBIR) project will test Cesium Iodide cluster ion beams as an alternative to lasers for desorbing bioions from a MALDI ((Matrix Assisted Laser Desorption Ionization) matrix. This offers the possiblity of performing the post source decay tandem mass spectrometry on an event by event basis, making possible the application of coincidence techniques which allow specific correlations to be made between metastable parent ions and their ionic fragments. This is an anaolgy to what has already been demonstrated for 252Cf fission fragment plasma desorption mass spectroscopy of bioions. Thus post source decay becomes a true MS/MS technique. There is also the possiblity that the use of cluster ions can increase the fragmentation and the degree of metastability of the desorbed bioions compared to using a laser. If so, the cluster deorption technique may be more suitable than lasers for imaging biomolecules on materials such as polymers or tissue slices. This Phase I project proposes to develop a cheap and reliable cluster ion source for MALDI. At the conclusion of Phase II, this ion source could be combined with a curve field reflector TOF mass spectrometer and marketed as a new approach to post source decay measurements.The commercial applications of this project will be in the area of biomolecular mass spectrometry.

这个小型企业创新研究第一阶段（SBIR）项目将测试碘化铯团簇离子束作为激光的替代品，用于从MALDI（基质辅助激光解吸电离）基质中解吸生物离子。这提供了在逐事件基础上执行源后衰变串联质谱的可能性，使得可以应用允许在亚稳母离子和它们的离子碎片之间进行特定相关性的符合技术。这是一个类比已经证明了252 Cf裂变碎片等离子体解吸质谱的生物离子。因此，源后衰变成为真正的MS/MS技术。与使用激光相比，使用簇离子还可能增加解吸附的生物离子的碎裂和亚稳态程度。如果是这样的话，簇脱附技术可能比激光更适合于在诸如聚合物或组织切片的材料上成像生物分子。第一阶段计画提出发展一种廉价且可靠的团簇离子源。在第二阶段结束时，该离子源可以与曲线场反射TOF质谱仪结合，作为一种新的源后衰变测量方法推向市场，该项目的商业应用将在生物分子质谱领域。