SBIR Phase I: Development of Integrated Fluid/Solid/Bio-Kinetic Simulation Software for the Characterization of Microsphere-based Bio-analytic Systems

SBIR 第一阶段：开发集成流体/固体/生物动力学模拟软件，用于表征基于微球的生物分析系统

• 批准号: 0109762
• 负责人: Shivshankar Sundaram
• 金额: $ 10万
• 依托单位: CFD RESEARCH CORPORATION
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0109762&HistoricalAwards=false
• 关键词: SBIR; Phase; Development; Integrated; Fluid

This Small Business Innovation Research Phase I (SBIR) project will demonstrate the feasibility and value of advanced simulation methodology for the prediction of biomolecular binding on the surface of microspheres used in biosensing applications. Models in current use employ many ad-hoc assumptions, particularly related to convective mass transport. Large, systematic errors are commonly encountered in predictive efforts. Novel, high-fidelity simulations proposed here hold the promise of providing a systematic understanding of the complex interaction between multiphase flow, diffusion and surface chemistry. An integrated simulation environment featuring Discrete Particle Simulations (suited for small beads) and Chimera Particle Simulations (for larger beads) will be developed in Phase I. Detailed bead-surface chemistry models (featuring finite-rate adsorption, desorption and conversion to irreversible state) will be developed and fully coupled to the flow model. The technique will be demonstrated using Immunoflow , a food bio-sensor based on fluidization developed at Utah State University. Both flow and binding simulation results will be validated against experiments. In Phase II, both Discrete and Chimera particle techniques will be further developed along with more generalized, user specifiable surface reaction mechanisms and model development for bio-molecule specific binding phenomena.The commercial application of the technology and the software to be developed in this project is in the area of biosensors for marketing to the biotechnology community. The product will enable the rapid creation of the next generation of optimized biosensors while simultaneously enhancing the fundamental understanding of biochemical processes. The technology would also benefit research in the traditional chemical and pharmaceutical industries.

这个小企业创新研究第一阶段（SBIR）项目将展示先进的模拟方法的可行性和价值，用于预测生物传感应用中使用的微球表面上的生物分子结合。目前使用的模型采用了许多特别的假设，特别是与对流质量传输有关的假设。在预测工作中经常会遇到大的系统性错误。新颖的，高保真度的模拟提出了这里的承诺，提供多相流，扩散和表面化学之间的复杂相互作用的系统的理解。第一阶段将开发一个集成的模拟环境，包括离散粒子模拟（适用于小珠）和嵌合体粒子模拟（适用于大珠）。将开发详细的珠表面化学模型（具有有限速率吸附、解吸和转化为不可逆状态），并将其与流动模型完全耦合。该技术将使用免疫流来演示，免疫流是一种基于犹他州州立大学开发的流化的食品生物传感器。流动和绑定模拟结果都将根据实验进行验证。在第二阶段，离散和嵌合粒子技术将得到进一步发展，沿着将有更广泛的、用户可指定的表面反应机制和生物分子特异性结合现象的模型发展。该技术的商业应用和将在该项目中开发的软件将在生物传感器领域向生物技术界销售。该产品将能够快速创建下一代优化的生物传感器，同时增强对生化过程的基本理解。该技术还将有利于传统化学和制药行业的研究。