Interference and NonInterference Recombination Pathways in Yeast

酵母中的干扰和非干扰重组途径

• 批准号: 0109809
• 负责人: Franklin Stahl
• 金额: $ 47.5万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-15 至 2006-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0109809&HistoricalAwards=false
• 关键词: Interference; NonInterference; Recombination; Pathways; Yeast

Evidence supports the following view of meiotic recombination in yeast: Double-strand-break repair is governed by two pathways, which are distinguished by the products of resolution of their repair intermediates. One pathway (Type I) yields the crossover and noncrossover resolution types expected from the cutting of both Holliday junctions of the intermediate. The other pathway (Type II) yields only one of the two types of crossover resolution that could arise from cutting DNA strands in the intermediate, along with a single noncrossover type that appears to arise without strand cutting. Crossover resolution of Type II intermediates depends on a set of proteins demonstrably required for crossover interference. Crossover resolution of Type I intermediates occurs independently of these proteins, and Type I crossovers display little or no interference. These studies of interference in yeast, which are expected to yield findings applicable to mammals and green plants as well, promise to provide an explanation for documented differences in crossing-over in yeast as compared with Drosophila. In so doing, they will enhance our understanding of meiosis, an essential feature of sexual reproduction. Understanding this basic biological process will enable biologists to develop better solutions to many of society's most pressing problems

证据支持以下酵母减数分裂重组的观点：双链断裂修复由两条途径控制，这两条途径由其修复中间体的分解产物区分。 一种途径（I型）产生交叉和非交叉分辨率类型预期从两个霍利迪交界处的中间切割。 另一种途径（II型）只产生两种类型的交叉分辨中的一种，这种交叉分辨可能是由于在中间体中切割DNA链而产生的，沿着的是一种非交叉类型，这种非交叉类型似乎是在没有链切割的情况下产生的。 II型中间体的交叉分离度取决于交叉干扰所需的一组蛋白质。 I型中间体的交叉分离独立于这些蛋白质发生，并且I型交叉显示很少或没有干扰。 这些对酵母的干扰研究，有望产生适用于哺乳动物和绿色植物的发现，有望为酵母与果蝇之间交换的差异提供解释。 这样做，他们将加强我们对减数分裂的理解，这是有性生殖的一个基本特征。了解这一基本的生物过程将使生物学家能够开发出更好的解决方案，以解决许多社会上最紧迫的问题