Molecular Genetic Dissection of Red/Far-Red Light Signaling Pathways in Maize

玉米红/远红光信号通路的分子遗传学解析

• 批准号: 0110297
• 负责人: Thomas Brutnell
• 金额: $ 35.05万
• 依托单位: Boyce Thompson Institute Plant Research
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0110297&HistoricalAwards=false
• 关键词: Molecular; Genetic; Dissection; Red; Far

Recent studies in the model plant Arabidopsis thaliana have greatly expanded our understanding of the complex light signal transduction networks mediating a range of developmental and environmental responses. However, as studies rapidly progress in Arabidopsis, our knowledge of this complex pathway in the distantly related monocots is far less complete. Differences in phytochrome gene family organization, plant physiology and selection pressures have likely contributed to the divergence of light signal transduction pathways between higher eudicots such as Arabidopsis thaliana and monocotyledonous grasses such as Zea mays. The goal of this project is to begin the detailed characterization of red/far-red light signaling pathways in maize utilizing classical and reverse genetics strategies, molecular genetic analyses and microarray technologies. A comprehensive analysis of phytochrome gene family structure and expression in maize will be conducted as an important first step in the analysis of light signaling pathways in maize. Through tools developed by NSF- and government-funding genomics initiatives, transposon-tagged alleles have been identified in two of the three phytochrome gene family members in maize. Genetic and molecular characterizations of these mutants will include the use of microarray technology to compare nuclear and chloroplast gene expression profiles in wild type and these phyA and phyB mutants under a range of light conditions. Finally, red/far-red light responses will be examined in a core set of 80 US, Canadian, European, and South American accessions that best represent diversity in the maize germplasm pool. These studies should provide insights into the underlying genetic diversity regulating light responses in maize.

最近在模式植物拟南芥中的研究极大地扩展了我们对介导一系列发育和环境响应的复杂光信号转导网络的理解。 然而，随着拟南芥研究的迅速进展，我们对远亲单子叶植物中这一复杂途径的了解还远未完全。光敏色素基因家族组织、植物生理和选择压力的差异可能导致了高等真双子叶植物如拟南芥和单子叶植物如玉米之间光信号转导途径的分歧。 该项目的目标是开始详细的表征红/远红光信号通路在玉米利用经典和反向遗传学策略，分子遗传学分析和微阵列技术。玉米光敏色素基因家族结构和表达的综合分析将作为玉米光信号通路分析的重要第一步。通过NSF和政府资助的基因组学计划开发的工具，转座子标记的等位基因已经在玉米的三个光敏色素基因家族成员中的两个中被鉴定出来。 这些突变体的遗传和分子特征将包括使用微阵列技术比较野生型和这些phyA和phyB突变体在一系列光照条件下的核和叶绿体基因表达谱。 最后，将在80个美国、加拿大、欧洲和南美种质的核心组中检查红光/远红光响应，这些种质最能代表玉米种质库的多样性。 这些研究将为深入了解调节玉米光反应的潜在遗传多样性提供帮助。