C-3'-Nucleic Acid Radicals: Generation and Mechanistic Investigations

C-3-核酸自由基：产生和机制研究

• 批准号: 0110588
• 负责人: Amanda Bryant-Friedrich
• 金额: $ 1.8万
• 依托单位: Oakland University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-15 至 2002-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0110588&HistoricalAwards=false
• 关键词: Nucleic; Acid; Radicals; Generation; Mechanistic

With this Minority Research Planning Grant Professor Bryant-Friedrich will gain knowledge and know-how related to the field of oxidative nucleic acid damage. She will also develop methodologies for the synthesis of modified nucleosides and nucleotides that function as C-3'-radical precursors in oligonucleotide systems. A foundation for the proposed research will be built by attending several conferences on radiation damage to DNA and on the biology of aging. These conferences will bring together practitioners in the field with expertise in chemistry, physics, biology and medicine. This will allow interaction with US and foreign researchers who investigate not only the mechanism of DNA damage but also those who study the actual induction process, the nature, structure, and distribution of DNA damage, as well as cellular effects and repair processes. The above interactions will be used as background information to establish a research program which focuses on the use of synthetic organic, physical organic, ahd bioorganic techniques for the independent generation of nucleoside radicals and the identification of the products that result from the degradation of these radical species. It will be necessary to synthesize precursors containing a wide variety of naturally occurring nucleobases as well as modified bases. These modified derivatives will be used in monomer experiments to determine the conditions necessary for efficient generation of the desired radicals, focusing on reaction times, solvents, proper derivatives, etc. When these details are established, further modification of the monomers will be carried out to facilitate their use in automated DNA synthesis. The work proposed by Professor Amanda Bryant-Friedrich in her proposal entitled "C-3'-Nucleic Acid Radicals: Generation and Mechanistic Investigations" is enthusiastically supported.

通过这项少数派研究计划拨款，布莱恩特·弗里德里希教授将获得与氧化核酸损伤领域相关的知识和技能。她还将开发合成修饰核苷和核苷酸的方法，这些核苷和核苷酸在寡核苷酸系统中充当 C-3' 自由基前体。将通过参加一些关于 DNA 辐射损伤和衰老生物学的会议来为拟议的研究奠定基础。这些会议将汇集该领域具有化学、物理、生物学和医学专业知识的从业者。这将允许与美国和外国研究人员进行互动，这些研究人员不仅研究 DNA 损伤的机制，还研究实际的诱导过程、DNA 损伤的性质、结构和分布，以及细胞效应和修复过程。上述相互作用将用作建立一个研究计划的背景信息，该计划的重点是使用合成有机、物理有机、ahd生物有机技术来独立生成核苷自由基，并鉴定这些自由基种类降解所产生的产物。有必要合成含有多种天然核碱基以及修饰碱基的前体。这些修饰的衍生物将用于单体实验，以确定有效生成所需自由基所需的条件，重点关注反应时间、溶剂、适当的衍生物等。当这些细节确定后，将对单体进行进一步修饰，以促进其在自动化 DNA 合成中的使用。 Amanda Bryant-Friedrich 教授在题为“C-3'-核酸自由基：生成和机理研究”的提案中提出的工作得到了热烈支持。