Microanalytical Methods for the Investigation of Metabolism and Transport of Substance P

由来自堪萨斯大学的合作者苏珊·M·伦特博士、肯尼斯·L·奥杜斯博士和凯伦·J·诺德海登博士共同完成的题为《用于研究P物质代谢和运输的微量分析方法》的研究项目得到了分析和表面化学计划的支持。该提案有四个具体目标：1)继续发展基于微芯片的分析。重点将是采用毛细管电泳法和电化学检测法(CEEC)的在线微透析。2)用微透析法和CEEC芯片监测体内微血管内皮细胞一氧化氮(NO)的释放。3)用铜络合和电化学检测继续研究P物质的运输和代谢。4)二维芯片分选系统的研制。该系统将用于P物质和其他多肽的预浓缩和分析。伦特在指导代表性不足的群体以及整合教育和研究方面享有盛誉。这项研究是多学科的，将对分离科学、基于微芯片的分析、神经传递和神经调节等领域产生重大影响。这项研究对后续医学进步有重大的潜在社会影响。

The research project by co-PI's Dr. Susan M. Lunte, Dr. Kenneth L. Audus and Dr. Karen J. Nordheden from the University of Kansas entitled "Microanalytical Methods for the Investigation of Metabolism and Transport of Substance P" is supported by the Analytical and Surface Chemistry Program. There are four specific aims in the proposal 1) Continued development in microchip based analysis. The emphasis will be toward on-line microdialysis with capillary electrophoresis and electrochemical detection (CEEC). 2) Monitoring nitric oxide (NO) release from microvessel endothelial cells in vivo using microdialysis and microchip CEEC. 3) Continued studies on the transport and metabolism of Substance P using copper complexation and electrochemical detection. 4) The development of a 2-dimensional microchip separation system. This system will be used for the preconcentration and analysis of substance P and other peptides.Dr. Lunte has an established reputation for mentoring underrepresented groups and for integrating education and research. This research is multidisciplinary and will have significant impact on the fields of separation science, microchip based analyses, neurotransmission and neuroregulation. There is significant potential societal impact from this research related to follow-on medical advances.