Microanalytical Methods for the Investigation of Metabolism and Transport of Substance P

该研究项目由共同PI的苏珊M。作者：Kenneth L. Audus和来自堪萨斯大学的Karen J. Nordheden博士题为“P物质代谢和运输研究的微量分析方法”的研究得到了分析和表面化学项目的支持。 该提案有四个具体目标：1）继续发展基于微芯片的分析。 重点将是对在线微透析与毛细管电泳和电化学检测（CEEC）。 2)利用微透析和微芯片CEEC监测体内微血管内皮细胞一氧化氮（NO）的释放。 3)利用铜络合和电化学检测继续研究P物质的转运和代谢。 4)二维微芯片分离系统的开发。 该系统将用于P物质和其他肽的预浓缩和分析。Lunte博士在指导代表性不足的群体和整合教育与研究方面享有盛誉。 这项研究是多学科的，将对分离科学，基于微芯片的分析，神经传递和神经调节领域产生重大影响。 这项研究对后续医学进步有重大的潜在社会影响。

The research project by co-PI's Dr. Susan M. Lunte, Dr. Kenneth L. Audus and Dr. Karen J. Nordheden from the University of Kansas entitled "Microanalytical Methods for the Investigation of Metabolism and Transport of Substance P" is supported by the Analytical and Surface Chemistry Program. There are four specific aims in the proposal 1) Continued development in microchip based analysis. The emphasis will be toward on-line microdialysis with capillary electrophoresis and electrochemical detection (CEEC). 2) Monitoring nitric oxide (NO) release from microvessel endothelial cells in vivo using microdialysis and microchip CEEC. 3) Continued studies on the transport and metabolism of Substance P using copper complexation and electrochemical detection. 4) The development of a 2-dimensional microchip separation system. This system will be used for the preconcentration and analysis of substance P and other peptides.Dr. Lunte has an established reputation for mentoring underrepresented groups and for integrating education and research. This research is multidisciplinary and will have significant impact on the fields of separation science, microchip based analyses, neurotransmission and neuroregulation. There is significant potential societal impact from this research related to follow-on medical advances.