Arabidopsis 2010: Identification of the Function of a Family of Putative Glycosyltransferases

拟南芥 2010：假定糖基转移酶家族功能的鉴定

• 批准号: 0114562
• 负责人: Christopher Somerville
• 金额: $ 69.3万
• 依托单位: Carnegie Institution of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0114562&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Identification; Function; Family

The goal of this project is to determine the biological function of genes which encode six families of structurally related proteins, called the cellulose synthase like (CSL) proteins. Based on the sequence similarity to cellulose synthase, it is hypothesized that the CSL genes encode processive glycosyltransferases that may catalyze the synthesis of some of the non-cellulosic polymers that comprise plant cell walls and other exopolysaccharides such as stylar secretions and mucilage, or the glycosyl residues on arabinogalactan proteins Because plant cell walls and exopolysaccharides are the dominant component of biomass on earth, knowledge of the enzymes and genes associated with the synthesis of these compounds may facilitate methods for the production and utilization of plant biomass.The technical approach that will be used to determine the function of the CSL genes exploits the recently completed full genome sequence of Arabidopsis to facilitate directed genetic analysis of the genes. Most of the CSL genes will be inactivated by insertional mutagenesis. In addition, the expression of the CSL genes will be altered by producing transgenic plants that have increased or decreased accumulation of mRNA for the CSL genes. The effects of the mutations and transgenic events on the growth and development of the plants, and on plant polysaccharide composition will be analyzed in order to associate each CSL gene with a specific biological role and enzyme of known catalytic activity. A list of the accession numbers of the genes to be analyzed during this project can be found at http://www.arabidopsis.org/info/2010_projects/index.html. Information about the gene families can be found at http://cellwall.stanford.edu/cesa/index.shtml.

该项目的目标是确定编码六个结构相关蛋白家族的基因的生物学功能，这些蛋白被称为纤维素合酶样（CSL）蛋白。基于与纤维素合酶的序列相似性，我们假设CSL基因编码的过程糖基转移酶可能催化一些非纤维素聚合物的合成，这些聚合物包括植物细胞壁和其他胞外多糖，如花柱分泌物和粘液，或阿拉伯半乳聚糖蛋白上的糖基残基，因为植物细胞壁和胞外多糖是地球上生物量的主要组成部分。了解与这些化合物合成相关的酶和基因可能有助于开发生产和利用植物生物量的方法。将用于确定CSL基因功能的技术方法利用最近完成的拟南芥全基因组序列来促进基因的定向遗传分析。大多数CSL基因将通过插入突变失活。此外，通过产生增加或减少CSL基因mRNA积累的转基因植株，可以改变CSL基因的表达。将分析突变和转基因事件对植物生长发育和植物多糖组成的影响，以便将每个CSL基因与特定的生物学作用和已知催化活性的酶联系起来。在这个项目中要分析的基因的加入号列表可以在http://www.arabidopsis.org/info/2010_projects/index.html上找到。有关基因家族的信息可以在http://cellwall.stanford.edu/cesa/index.shtml上找到。