Regulation of fatty acid desaturation in Arabidopsis

拟南芥中脂肪酸去饱和的调控

• 批准号: 9219356
• 负责人: Christopher Somerville
• 金额: $ 8.5万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9219356&HistoricalAwards=false
• 关键词: Regulation; fatty; acid; desaturation; Arabidopsis

The goal of the project is to elucidate the molecular mechanisms which regulate the degree of unsaturation of membrane and storage lipids. We will address this question by measuring the effect of variation in developmental stage and environmental conditions on the expression of several recently isolated genes encoding fatty acid desaturases from the plant Arabidopsis thaliana. The existence of regulatory mechanisms which act at the level of direct enzyme activation or deactivation will be examined by measuring the effects on lipid composition in transgenic plants which contain higher than normal levels of desaturase activity due to overexpression of cloned desaturase genes. The spatial organization of lipid desaturation within cells will be elucidated using anti-desaturase antibodies to localize the enzymes by immunogold electron microscopy. Finally, we will attempt to isolate genes for several additional desaturases by map-based cloning methods based on the availability of a comprehensive collection of mutants of Arabidopsis. %%% Plant fatty acids provide about one third of the calories in human diets and are also used for a wide variety of technical products raging from synthesis of nylon to cosmetics. In addition, the ability of many plants to withstand exposure to temperature extremes is thought to be controlled by the fatty acid composition of plant membranes. The experiments proposed here are designed to provided detailed information about the factors which regulate plant fatty acid composition. This information may be useful in permitting genetic modification of the fatty acid composition of plants so that they produce edible oils with advantageous dietary qualities, expanded technical uses or enhanced ability to withstand temperature extremes.

该项目的目标是阐明分子机制 其调节膜的不饱和度和储存 脂质。 我们将通过测量 发育阶段和环境条件的变化 最近分离的几个编码脂肪的基因的表达 来自植物拟南芥的酸性去饱和酶。 的 在直接一级发挥作用的管理机制的存在 酶的激活或失活将通过测量 对转基因植物中脂质组成的影响， 去饱和酶活性高于正常水平， 克隆的去饱和酶基因的过表达。 空间 将阐明细胞内脂质去饱和的组织 使用抗去饱和酶抗体定位酶， 免疫金电镜 最后，我们将尝试 通过基于图位分离几种另外去饱和酶的基因 克隆方法的基础上，全面的可用性 拟南芥突变体的集合。 %%% 植物脂肪酸提供了人体约三分之一的热量 也用于各种技术产品 从尼龙合成到化妆品。 此外该 许多植物能经受住暴露在温度下的能力 极端被认为是由脂肪酸组成控制 植物膜。 这里提出的实验旨在 提供了有关调节因素的详细信息， 植物脂肪酸组成。 这些信息可能有助于 允许遗传修饰的脂肪酸组成， 植物，以便它们生产具有有利的膳食营养的食用油， 质量，扩大技术用途或增强承受能力， 极端温度