Optical Spectroscopy in the Undergraduate Laboratory Curriculum

生物科学（61）利用从文献改编的光谱方法的实验正在以协调的方式引入四个教学实验室，作为提高学生对光和物质相互作用的理解的一种手段。学生在一年级无机化学实验室测量金属卟啉的吸光度和荧光。他们表征蛋白质-DNA相互作用，DNA-配体热力学和动力学，以及蛋白质展开平衡和动力学在上层生物化学和化学实验室使用吸收和荧光光谱。在一个实验室课程，学生开发和测试自己的假设一个明确的生物物理问题。目前正在开发和实施基于网络的教学工具，以加强和监测学生的学习。促进这些活动的仪器包括一个紫外可见分光光度计，两个扫描荧光计（与计算机/打印机），和一个快速混合配件停流动力学专用于本科教学实验室。该项目正在由化学和生物系的咨询委员会以及一名教学专家进行评估。

Biological Sciences (61)Experiments that utilize optical spectroscopic methods adapted from the literature are being introduced in a coordinated fashion into four teaching laboratories as a means of increasing student understanding of interactions of light and matter. Students measure the absorbance and fluorescence of metalloporphyrins in a freshman-level inorganic chemistry laboratory. They characterize protein-DNA interactions, DNA-ligand thermodynamics and kinetics, and protein unfolding equilibrium and kinetics in upper-level biochemistry and chemistry laboratories using absorbance and fluorescence spectroscopy. In one laboratory course, students develop and test their own hypothesis for a well-defined biophysical problem. Web-based teaching tools are being developed and implemented to enhance and monitor student learning. Instrumentation to facilitate these activities include a UV-visible spectrophotometer, two scanning fluorometers (with computers/printers), and a rapid-mixing accessory for stopped-flow kinetics for dedicated use in the undergraduate teaching laboratories. This project is being assessed by an advisory board of chemistry and biology faculty and a teaching and learning specialist.