High Throughput Sequencing and the Phylogeny of Family Drosophilidae

高通量测序和果蝇科的系统发育

• 批准号: 0129105
• 负责人: Robert DeSalle
• 金额: $ 36.72万
• 依托单位: American Museum Natural History
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0129105&HistoricalAwards=false
• 关键词: Throughput; Sequencing; Phylogeny; Family; Drosophilidae

DEB-0129105R. DeSalle & P. O'GradyA grant has been awarded to Drs. Rob DeSalle and Patrick O'Grady of the American Museum of Natural History to investigate evolutionary relationships of flies in the family Drosophilidae, a group of about 3,300 described species. In spite of recent research efforts, systematic relationships of genera and species groups within the Drosophilidae remain unresolved. This is likely due to the rapid diversification of this large group. The proposed work will generate about 60 gene sequences from over 100 species. This will comprise one of the largest collections of nucleotide data, in terms of number of loci and taxa, ever gathered for a phylogenetic study. In addition to being applied to understanding the phylogenetic relationships within the Drosophilidae, these data will also be used to examine the molecular evolutionary patterns and processes acting on the genomes of these species.The Drosophilidae has served as a model system in genetics, evolution, and development for over one hundred years. The recent completion of the Drosophila melanogaster genome sequence, provides us with an unprecidented amount of data which can be used to initiate comparative studies in other drosophild species. The proposed work will make the entire Drosophilidae a model system for molecular phylogenetic studies. Furthermore, the data generated in this proposed work will facilitate the next stage of comparative evolutionary studies - comparative genomics.

DEB-0129105R。美国自然历史博物馆的罗布·德萨尔和帕特里克·奥格雷迪博士获得了一笔赠款，用于研究果蝇科中苍蝇的进化关系。果蝇科是一个约有3,300种被描述的物种。尽管最近的研究努力，果蝇科内的属和种组的系统关系仍然没有得到解决。 这可能是由于这个庞大集团的迅速多样化。 这项拟议的工作将产生来自100多个物种的大约60个基因序列。 这将包括一个最大的收集核苷酸数据，在基因座和类群的数量，以往任何时候都收集了系统发育研究。 这些数据除了用于了解果蝇科的系统发育关系外，还将用于研究作用于这些物种基因组的分子进化模式和过程。果蝇科作为遗传学、进化和发展的模型系统已有一百多年的历史。 最近完成的果蝇基因组序列，为我们提供了一个前所未有的数据量，可用于启动在其他果蝇物种的比较研究。 这项工作将使整个果蝇科成为分子系统发育研究的模式系统。 此外，在这项拟议的工作中产生的数据将促进下一阶段的比较进化研究-比较基因组学。