Multitrophic Interactions with Oaks

植物的功能取决于有益和有害的生物营养相互作用与内部过程的相互作用，例如，在橡树中观察到的茎和根交替生长的内源节律发育。在本项目中，四个申请小组将比较面对主要生物营养伙伴的橡树的基因调控和资源分配，即外生菌根、根致病微生物、根际无脊椎动物消费者、菌根化辅助细菌、叶致病真菌和叶无脊椎食草动物。通过采用13C和15N的示踪实验，以及采用qRT-PCR完成的30 000 EST芯片的转录组学分析，将克隆菌根橡树微扦插的联合实验平台用于分析标准化条件下每个生物营养伴侣的影响。在其他实验中，项目部分将结合不同的生物营养剂，考虑联系的动态，地上地下的相互作用或气候条件的影响。该项目旨在解开相互作用的共同或特定的调节机制，启动受控多奖杯实验，并构建主题微阵列。后续的研究单位将深化在不同环境条件下，多肥育对植物结果影响的调控研究和实地分析

Plant functioning depends on beneficial and detrimental biotrophic interactions intermingled with internal processes, e.g. the endogenous rhythmic development with alternating shoot and root growth flushes observed in oak trees. In this project four applicant groups will compare gene regulation and resource allocation in oaks faced with main biotrophic partners, i.e. ectomycorrhiza, root pathogenic microorganisms, rhizospheric invertebrate consumers, mycorrhization helper bacteria, leaf pathogenic fungi and leaf invertebrate herbivores. A Joined Experimental Platform with clonal mycorrhizal oak microcuttings will serve to analyse the impact of each biotrophic partner under standardized conditions by tracer experiments with 13C and 15N and transcriptomic with a 30 000 EST microarray completed by qRT-PCR. In additional experiments the project parts will combine different biotrophic agents, consider the dynamics of the associations, above-belowground interactions or the influence of climatic conditions. The project aims at disentangling regulation mechanisms common or specific of the interactions, initiating controlled multitrophy experiments, and constructing a thematic microarray. A follow-up Research Unit will deepen regulation studies and field analyses on the impact of multitrophy on the plant outcome under different environmental conditions