Multitrophic Interactions with Oaks

植物的功能取决于有益和有害的生物营养相互作用与内部过程的混合，例如在橡树中观察到的具有交替的茎和根生长冲洗的内源性节律性发育。在这个项目中，四个申请组将比较基因调控和资源分配的橡树面临的主要生物营养伙伴，即外生菌根，根病原微生物，根际无脊椎动物消费者，菌根化辅助细菌，叶病原真菌和叶无脊椎动物食草动物。一个联合实验平台与克隆菌根橡树微插条将用于分析每个生物营养伙伴的影响，在标准化条件下，通过示踪实验与13 C和15 N和转录组与30 000 EST微阵列完成qRT-PCR。在额外的实验中，项目部分将结合联合收割机不同的生物营养剂，考虑协会的动态，地上地下的相互作用或气候条件的影响。该项目旨在解开相互作用的共同或特定的调节机制，启动受控的多营养实验，并构建主题微阵列。后续研究组将深入研究在不同环境条件下多营养对植物产量的影响

Plant functioning depends on beneficial and detrimental biotrophic interactions intermingled with internal processes, e.g. the endogenous rhythmic development with alternating shoot and root growth flushes observed in oak trees. In this project four applicant groups will compare gene regulation and resource allocation in oaks faced with main biotrophic partners, i.e. ectomycorrhiza, root pathogenic microorganisms, rhizospheric invertebrate consumers, mycorrhization helper bacteria, leaf pathogenic fungi and leaf invertebrate herbivores. A Joined Experimental Platform with clonal mycorrhizal oak microcuttings will serve to analyse the impact of each biotrophic partner under standardized conditions by tracer experiments with 13C and 15N and transcriptomic with a 30 000 EST microarray completed by qRT-PCR. In additional experiments the project parts will combine different biotrophic agents, consider the dynamics of the associations, above-belowground interactions or the influence of climatic conditions. The project aims at disentangling regulation mechanisms common or specific of the interactions, initiating controlled multitrophy experiments, and constructing a thematic microarray. A follow-up Research Unit will deepen regulation studies and field analyses on the impact of multitrophy on the plant outcome under different environmental conditions