Multitrophic Interactions with Oaks

植物的功能依赖于有益和有害的生物营养相互作用与内部过程的混合，例如，在橡树中观察到的内源节律发育伴随着交替的地上部和根部生长。在这个项目中，四个申请小组将比较橡树面临的主要生物营养伙伴的基因调控和资源分配，即外生菌根、根部病原微生物、根际无脊椎动物消费者、菌根化辅助细菌、叶片病原真菌和叶片无脊椎动物草食动物。通过13C和15N的示踪实验，以及利用qRT-PCR完成的30000个EST微阵列的转录实验，建立了一个克隆菌根橡树微切割实验平台，用于分析标准化条件下每个生物营养伙伴的影响。在额外的实验中，项目部分将结合不同的生物营养剂，考虑联合的动态，地上和地下的相互作用或气候条件的影响。该项目旨在解开相互作用的共同或特定的调控机制，启动受控的多营养体实验，并构建主题微阵列。后续研究单位将深化关于不同环境条件下多重营养对植物结果的影响的调节研究和田间分析

Plant functioning depends on beneficial and detrimental biotrophic interactions intermingled with internal processes, e.g. the endogenous rhythmic development with alternating shoot and root growth flushes observed in oak trees. In this project four applicant groups will compare gene regulation and resource allocation in oaks faced with main biotrophic partners, i.e. ectomycorrhiza, root pathogenic microorganisms, rhizospheric invertebrate consumers, mycorrhization helper bacteria, leaf pathogenic fungi and leaf invertebrate herbivores. A Joined Experimental Platform with clonal mycorrhizal oak microcuttings will serve to analyse the impact of each biotrophic partner under standardized conditions by tracer experiments with 13C and 15N and transcriptomic with a 30 000 EST microarray completed by qRT-PCR. In additional experiments the project parts will combine different biotrophic agents, consider the dynamics of the associations, above-belowground interactions or the influence of climatic conditions. The project aims at disentangling regulation mechanisms common or specific of the interactions, initiating controlled multitrophy experiments, and constructing a thematic microarray. A follow-up Research Unit will deepen regulation studies and field analyses on the impact of multitrophy on the plant outcome under different environmental conditions