Genetics of Haldane's Rule in Caenorhabditis

霍尔丹法则在秀丽隐杆线虫中的遗传学

• 批准号: 0130987
• 负责人: Scott Baird
• 金额: $ 25.79万
• 依托单位: Wright State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0130987&HistoricalAwards=false
• 关键词: Genetics; Haldane; Rule; Caenorhabditis

Haldane's rule is the observation that "when in the F1 offspring of two different animals races one sex is absent, rare, or sterile, that sex is the heterozygous [heterogametic] sex" (Haldane, 1922). In the nematode genus Caenorhabditis, Haldane's Rule is observed in C. briggsae::C. remanei hybrids. Implementation in this combination is through sexual transformation of haplo-X hybrids into females. Hybrid sexual transformation likely results from dysgenic interactions among sex determination genes. This hypothesis will be tested by mapping naturally occurring variants that suppress the hybrid sexual transformation phenotype relative to the sex determination genes of C. briggsae and C. remanei. One such variant has been identified in each of these species. Mapping of sexual transformation variants will utilize single-nucleotide polymorphisms(SNPs) as genetic markers. The goal of this project is to map hybrid sexual transformation variants to the nucleotide. This will be accomplished in two stages. A resolution of 0.1 mu (0.1% recombination) will be achieved by analysis of recombinant chromosomes through a single-worm PCR assay. Depending on the genomic region involved, this resolution will correspond to between 5,000 to 150,000 bp. Increased resolution (0.0001 mu) will be achieved through a population-based assay designed to detect recombination between adjacent SNPs. This work will further the understanding of the evolutionary genetics of species formation.--

霍尔丹规则是这样的观察：“当两个不同动物种族的 F1 后代中，一种性别不存在、稀有或不育时，该性别就是杂合[异配]性别”（Haldane，1922）。 在线虫属 Caenorhabditis 中，在 C. briggsae::C 中观察到霍尔丹法则。雷马内伊杂交种。 这种组合的实现是通过将 haplo-X 杂种有性转化为雌性。 混合性转变可能是由性别决定基因之间的基因失调相互作用引起的。 该假设将通过绘制天然存在的变异体进行测试，这些变异体抑制与 C. briggsae 和 C. remanei 的性别决定基因相关的杂种有性转化表型。 在这些物种中的每一个中都发现了一种这样的变异。 性转化变异的绘图将利用单核苷酸多态性（SNP）作为遗传标记。 该项目的目标是将杂交性转化变异映射到核苷酸上。 这将分两个阶段完成。 通过单线虫 PCR 检测对重组染色体进行分析，可实现 0.1 mu（0.1% 重组）的分辨率。根据所涉及的基因组区域，该分辨率将对应于 5,000 至 150,000 bp。 分辨率的提高（0.0001 mu）将通过基于群体的检测来实现，该检测旨在检测相邻 SNP 之间的重组。 这项工作将进一步加深对物种形成的进化遗传学的理解。--