Acqusition of Genonics Instrumentation for Evolutionary, Ecological, and Environmental Biology.

该合同将支持收购基因组学仪器，包括高通量实时聚合酶链式反应和微阵列打印和扫描。这些工具将加强以下项目：(1)适应基因复合体的演变(2)昆虫取食和其他环境应激源对植物全球基因表达的影响，(3)人工湿地微生物和根群落结构的分析，以及(4)蓝藻毒素的毒理基因组学和有害藻华的基因组和蛋白质组特征。这项提议的目标是建立一个基因组学设施，用于对非模型生物的研究，这些生物是莱特州立大学正在进行的研究项目的主题。非模式生物的使用是进化、生态和环境生物学研究的一个重要方面。基因组学彻底改变了生物科学的研究方式。基因组学技术在环境和生态研究中的应用有可能给这些领域带来革命性的变化，因为它已经彻底改变了分子和细胞生物学。例如，该奖项促成的一个项目是对为处理四氯乙烯及其致癌降解产物氯乙烯而建造的湿地中的细菌群落进行分析。该湿地中细菌群落结构和演替的特征将有助于设计用于处理其他有机化合物的湿地。另一个项目是对藻类水华进行基因组分析，这将使人们能够快速区分有毒和无毒的水华。第三个项目将需要开发利用单核苷酸多态进行遗传图谱绘制的高通量方法。收购仪器的技术创新将被整合到研究生和本科生教育项目中，包括针对代表性不足群体的项目。该领域最近的努力包括资助环境生物学本科生辅导项目(UMEB)，为代表不足的人开展环境压力研究，以及为残疾理科学生创建实验室准入(CLASS)。这些学生中的许多人继续在生物科学系的研究生课程中学习。其中一个是2002年建立的环境科学博士项目。环境科学是莱特州立大学的一个杰出领域。

This award will support the acquisition of genomics instrumentation including high-throughput real-time PCR and microarray printing and scanning. These instruments will enhance projects that include the following: (1) the evolution of adaptive gene complexes (2) the effects of insect feeding and other environmental stressors on global gene expression in plants, (3) analyses of microbial and root community structures in constructed wetlands, and (4) toxicogenomics of cyanobacterial toxins and genomic and proteomic characterizations of harmful algal blooms. The goal of this proposal is the establishment of a genomics facility that will be used for research on nonmodel organisms that are the subject of ongoing research programs at Wright State University. The use of nonmodel organisms is a crucial aspect in studies of evolutionary, ecological, and environmental biology.Genomics has revolutionized the way research is conducted in Biological Sciences. Application of genomics technology to environmental and ecological studies has the potential to revolutionize these fields as it has revolutionized molecular and cellular biology. For example, one project made possible by this award is the analysis of bacterial communities in a wetland constructed for the treatment of perchloroethylene and its carcinogenic degradation product, vinyl chloride. Characterization of bacterial community structure and succession in this wetland will help in the design of wetlands constructed for the treatment of other organic compounds. Another project will be genomic analyses of algal blooms, which will allow for rapid discrimination between toxic and nontoxic blooms. A third project will entail the development of high throughput methods for genetic mapping using single nucleotide polymorphisms. The technical innovations from the acquired instrumentation will be integrated into graduate and undergraduate educational programs, including programs that target underrepresented groups. Recent efforts in this area include funded projects for Undergraduate Mentoring in Environmental Biology (UMEB), Research in Environmental Stress for the Underrepresented, and Creating Laboratory Access for Science Students with Disabilities (CLASS). Many of these students continue their education in the graduate programs of the Department of Biological Sciences. Among these is an Environmental Sciences Ph.D. program that was established in 2002. Environmental Sciences is an area of excellence at Wright State.