CAREER: Lateral Phase Separation of Rafts and Liquid Domains in Lipid Systems

职业：脂质系统中筏和液体域的横向相分离

• 批准号: 0133484
• 负责人: Sarah Keller
• 金额: $ 64.5万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0133484&HistoricalAwards=false
• 关键词: CAREER; Lateral; Phase; Separation; Rafts

This CAREER award supports research in the characterization of "raft" domains in lipid monolayers and bilayers. Rafts are regions in cell membranes in which particular lipids and proteins are concentrated, and can play an important role in biological functions such as signaling. This research will test a central hypothesis that the phase behavior of raft domains in lipid vesicles can be predicted from the phase behavior of the same lipids in monolayers. Specific goals of this research include using fluorescence microscopy and 2-photon microscopy to identify raft domains in giant unilamellar vesicles using lipids known to form liquid ordered domains in monolayers. In addition, the research will test whether changing symmetry from a monolayer to a bilayer alters domain formation. This will be evaluated by searching for raft domains in monolayers deposited on a surface patterned with silanes or alkane-thiols. Long-term goals include assessing whether the presence of rafts alters the activity of membrane proteins.Education efforts are focussed primarily on improving mathematical literacy of chemistry and biochemistry students. The importance of mathematics will be advertised to these students early in their college careers. A mathematics self-tutorial will be developed to clarify areas in which students need help. Resultant data will be used to guide curriculum changes. Study groups and e-mail threads will be instituted to help students learn from each other. Effort will also be directed to increasing departmental involvement of graduate students through a student-run colloquium series, and training students in the laboratory.

该CAREER奖支持脂质单层和双层中“筏”结构域的表征研究。 筏是细胞膜中的区域，其中集中了特定的脂质和蛋白质，并且可以在信号传导等生物功能中发挥重要作用。 这项研究将测试一个中心的假设，筏结构域的相行为的脂质囊泡可以预测的相行为相同的脂质在单层。本研究的具体目标包括使用荧光显微镜和2-光子显微镜，以确定筏结构域在巨大的单层囊泡使用脂质已知形成液体有序结构域的单层。 此外，该研究还将测试从单层到双层的对称性变化是否会改变域的形成。 这将通过在沉积在用硅烷或烷硫醇图案化的表面上的单层中搜索筏域来评估。 长期目标包括评估筏的存在是否改变膜蛋白的活性。教育工作主要集中在提高化学和生物化学学生的数学素养上。数学的重要性将在这些学生大学生涯的早期就被宣传。 将开发一个数学自学教程，以澄清学生需要帮助的领域。 由此产生的数据将用于指导课程改革。 将设立学习小组和电子邮件线程，以帮助学生相互学习。 还将努力通过学生主持的系列座谈会和在实验室培训学生来增加研究生的部门参与。