CAREER: Roles of hippocampal/neostriatal systems in multiple forms of memory

摘要提案编号：IBN-0133734 PI姓名：Paul colombo该职业发展计划整合了杜兰大学促进神经科学研究和教育的使命背景下的教学，指导和研究目标。教育目标包括为新的神经科学本科专业开发讲座和实验课程，并从传统上在神经科学领域代表性不足的群体中招募本科生，在夏季参加研究培训实习。这个项目广泛的研究目标是系统地定义两个已知支持哺乳动物不同类型记忆的大脑区域之间的关系：新纹状体和海马体。虽然这两个系统的作用已经在实验中分离，但它们独立、合作、竞争或时间顺序的作用程度目前尚不清楚。此外，这两种系统支持记忆形成的时间过程和细胞机制尚不清楚。记忆的形成伴随着大脑中参与信息处理的神经元的区域特异性变化。这些变化是记忆和大脑区域特异性的假设将被测试：新纹状体激活支持反应、非空间和程序记忆，而海马体激活支持位置、空间和陈述性记忆。目前的提案采用了两种主要的研究策略。第一种策略是使用行为操纵来诱导记忆形成的细胞机制中与学习相关的变化，并量化新纹状体和海马形成中的这些变化。第二个研究策略是测试高选择性蛋白质抑制对新纹状体和海马依赖性记忆的获得和保留的影响。通过这些研究策略的结合，将在行为、脑系统和神经元水平的分析中阐明海马体形成和新纹状体之间的关系。除了本提案的研究目标之外，还有两个额外的目标都涉及研究、教学和指导的整合。这些目标中的第一个是增加神经科学教育和研究经验的机会，这些学生来自传统上在神经科学领域代表性不足的群体，方法是在夏季在实验室指导学生，并对该地区的大学进行外联访问。第二个目标是继续发展杜兰大学的神经科学本科专业。

LAY ABSTRACT Proposal # : IBN-0133734 PI Name: Paul ColomboThis career development plan integrates teaching, mentoring, and research goals within the context of Tulane University's mission to promote neuroscience research and education. The educational goals include development of lecture and laboratory curricula for the new undergraduate neuroscience major, and recruitment of undergraduate students from groups traditionally underrepresented in the neurosciences to participate in research training internships during the summer months. The broad research goal of this project is to systematically define relationships between two brain regions known to support different types of memory among mammalian species: the neostriatum and the hippocampal formation. Although the roles of these two systems have been dissociated experimentally, the extent to which they function independently, cooperatively, competitively, or in temporal sequence is not known currently. Moreover, the time courses and cellular mechanisms of memory formation supported by these two systems are not well understood. Memory formation is accompanied by brain region-specific changes among neurons involved in information processing. Hypotheses that these changes are memory- and brain region-specific will be tested: neostriatal activation supports response, nonspatial, and procedural memory, whereas hippocampal activation supports place, spatial, and declarative memory. The current proposal employs two primary research strategies. The first strategy is to use behavioral manipulations to induce learning-related changes in cellular mechanisms of memory formation and to quantify those changes within the neostriatum and hippocampal formation. The second research strategy is to test the effects of highly selective protein inhibition on acquisition and retention of neostriatal- and hippocampal-dependent memory. By combining these research strategies, relationships between the hippocampal formation and neostriatum will be elucidated during multiple types of memory formation at the behavioral, brain systems, and neuronal levels of analyses.In addition to the research goals of this proposal, two additional goals both involve integration of research, teaching, and mentoring. The first of these goals is to increase opportunities for neuroscience education and research experiences for students from groups traditionally underrepresented in the neurosciences by mentoring students in the laboratory during the summer, and by outreach visits to colleges in the area. The second goal is to continue to develop the undergraduate major in Neuroscience at Tulane University.