RUI: Molecular Phylogenetics and Biogeography of the Poison Frogs (Family Dendrobatidae)

RUI：毒蛙（Dendrobatidae 科）的分子系统发育学和生物地理学

• 批准号: 0134191
• 负责人: Kyle Summers
• 金额: $ 14.65万
• 依托单位: East Carolina University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0134191&HistoricalAwards=false
• 关键词: RUI; Molecular; Phylogenetics; Biogeography; Poison

This award is to fund an investigation of the systematics and biogeography of the poison frogs (family Dendrobatidae) in Peru and other parts of Amazonia. The award will fund the collection of specimens in the field, and the analysis of DNA from tissue samples in the laboratory. Tissue samples (toe clips) will be collected from poison frogs from remote parts of the Peruvian rainforest. These and previously obtained samples from other regions of Amazonia will be used to obtain DNA sequences from six gene regions via molecular genetic analysis. The DNA sequence variation among populations and species will be used in a phylogenetic analysis of the systematic relationships of the poison frog family. Information on DNA sequence variation among populations will also be used to investigate associations between genetic divergence and geographic barriers to gene flow in Amazonia. Finally, the information obtained on the systematic relationships of the poison frogs will be used to investigate evolutionary associations between life history and ecological characteristics of the poison frogs, including parental care, egg and clutch size, tadpole deposition pool size, tadpole cannibalism, mating systems, and sexual dimorphism. This research will provide the first thorough analysis of systematic relationships in the poison frog family. It will also provide an important contribution to recent debates about why the Amazon River basin supports such a huge number of species. Most of these debates have centered on the contribution of specific geographic barriers to genetic divergence, and this research will directly address the relevant hypotheses. The systematic results from this research will be used to investigate important questions concerning the evolutionary ecology of the poison frogs in a comparative framework.

该奖项将资助对秘鲁和亚马逊其他地区毒蛙（石蛙科）的系统和生物地理学研究。该奖项将资助在该领域收集标本，并在实验室对组织样本进行DNA分析。组织样本（脚趾夹）将从秘鲁热带雨林偏远地区的毒蛙身上收集。这些和先前从亚马逊其他地区获得的样本将用于通过分子遗传分析从六个基因区域获得DNA序列。在种群和种间的DNA序列变异将用于毒蛙科系统关系的系统发育分析。关于种群间DNA序列变异的信息也将用于调查亚马孙地区遗传分化与基因流动的地理障碍之间的关系。最后，研究结果将用于研究毒蛙的生活史和生态特征之间的进化关系，包括亲代抚育、卵卵数量、蝌蚪沉积池大小、蝌蚪同类相食、交配系统和两性二态性。这项研究将首次全面分析毒蛙家族的系统关系。它也将为最近关于亚马逊河流域为什么支持如此大量物种的争论提供重要的贡献。这些争论大多集中在特定地理障碍对遗传分化的贡献上，而本研究将直接解决相关假设。本研究的系统结果将用于在比较框架中研究有关毒蛙进化生态学的重要问题。