Molecular Phylogenetics, Diagnostics and Control of Apicomplexan Parasites
顶复门寄生虫的分子系统发育、诊断和控制
基本信息
- 批准号:RGPIN-2016-05826
- 负责人:
- 金额:$ 2.4万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2018
- 资助国家:加拿大
- 起止时间:2018-01-01 至 2019-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The*long term goal of my research program is to explore and understand the interactions between*parasites of the phylum Apicomplexa and their hosts at the historical,*organismal (host-parasite) and molecular levels. These parasites cause*important animal and human diseases such as malaria, toxoplasmosis,*cryptosporidiosis and coccidiosis. I propose to further our understanding of*these relationships by pursuing three main areas of study:***1) Investigate apicomplexan phylogeny and host-parasite coevolution*using multi genome molecular markers with morphological and morphometric*characters: We will determine the biodiversity of apicomplexan parasites in*humans, domesticated animals and wildlife (potential zoonotics) by generating*sequence data (nuclear 18S rDNA, plastid rDNA/polymerase B, and mt cytochrome c oxidase subunit I, COI) from*apicomplexan parasites that infect both domestic and, largely ignored, wildlife*hosts. Our newly developed PCR primer pairs will generate species-specific*COI-based DNA barcodes that will be used to identify specifically coccidia*(Eimeriorina) including enteric (e.g. Eimeria)*and tissue (e.g. Toxoplasma)*coccidia. We will modify our DNA barcode primers for use in a multiplexed NGS*assay to both identify and enumerate mixed coccidia in biological samples such*as fecal or tissue samples; this method will permit rapid protistan parasite*biodiversity and species discovery;***2)*Generate complete mitochondrial (mt) genomes from biologically diverse*apicomplexan parasites to explore the structural evolution of mt genomes and*generate sequence data necessary for inferring the evolutionary history of*these cosmopolitan parasites.***3) Explore immunological control of coccidia of domestic poultry. We*will determine*the natural prevalence of Eimeria that infect domestic turkeys*nationally and internationally to determine the number of species required in a*multivalent live coccidiosis vaccine against turkey coccidiosis. We will refine and further develop a recombinant*multivalent vaccine technology that combines two parasite antigenic epitopes with a*host-derived immune modulating epitope (HMGB1). *The current Bacillus subtilis expression vector for this multivalent*vaccine construct will be converted into a strictly peptide based (i.e.*chemically synthesized) form and tested for biological activity in vivo when*delivered by various means. Successful*vaccination against Eimeria species using a non-viable vaccine construct (a*global first) would address vaccine production, transportation and shelf life*issues as well as avoid health implications of using live coccidiosis vaccines*in commercial poultry.**
我的研究计划的长期目标是探索和理解顶复门的寄生虫和它们的宿主之间的相互作用在历史上,生物体(宿主-寄生虫)和分子水平。这些寄生虫引起重要的动物和人类疾病,如疟疾、弓形虫病、隐孢子虫病和球虫病。我建议通过以下三个主要研究领域来进一步理解这些关系:*1)使用具有形态和形态测量 * 特征的多基因组分子标记来研究顶复门的发育和宿主-寄生虫的共同进化 *:我们将确定人类、家养动物和野生动物中顶复门寄生虫的生物多样性(潜在的人畜共患病)通过生成 * 序列数据(核18 S rDNA,质体rDNA/聚合酶B,和mt细胞色素c氧化酶亚基I,COI)从顶复门寄生虫,感染国内和很大程度上被忽视,野生动物宿主。我们新开发的PCR引物对将产生物种特异性 * 基于COI的DNA条形码,该条形码将用于特异性鉴定球虫 *(艾美球虫属),包括肠球虫(例如艾美球虫)* 和组织球虫(例如弓形虫)*。我们将修改我们的DNA条形码引物,用于多重NGS* 检测,以识别和计数生物样品(如粪便或组织样品)中的混合球虫;这种方法将允许快速原生生物寄生虫生物多样性和物种发现;*2)* 从生物多样性 * 顶复门寄生虫中生成完整的线粒体(mt)基因组,以探索mt基因组的结构进化,以及 * 生成推断这些世界性寄生虫进化历史所需的序列数据。3)家禽球虫免疫控制的探讨。我们将在国内和国际上确定感染火鸡的艾美耳球虫的自然流行率,以确定针对火鸡球虫病的多价活球虫病疫苗所需的物种数量。我们将改进和进一步开发一种重组多价疫苗技术,该技术将两种寄生虫抗原表位与一种宿主衍生的免疫调节表位(HMGB 1)结合起来。* 用于该多价疫苗构建体的当前枯草芽孢杆菌表达载体将被转化为严格基于肽的表达载体(即,化学合成)形式,并在通过各种方式递送时测试体内生物活性。 使用非活疫苗构建体成功 * 接种艾美球虫属物种(* 全球第一)将解决疫苗生产、运输和保质期 * 问题,并避免在商业家禽中使用活球虫病疫苗 * 的健康影响。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Barta, John其他文献
Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States.
- DOI:
10.1016/j.psj.2023.102819 - 发表时间:
2023-08 - 期刊:
- 影响因子:4.4
- 作者:
Trujillo-Peralta, Carolina;Ashcraft, Aaron;Senas-Cuesta, Roberto;Coles, Makenly;Hernandez-Velasco, Xochitl;Selby, Callie;Forga, Aaron;Tellez-Isaias, Guillermo;Vuong, Christine;Bielke, Lisa;Barta, John;Hargis, Billy;Graham, Danielle - 通讯作者:
Graham, Danielle
Barta, John的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Barta, John', 18)}}的其他基金
Molecular Phylogenetics, Diagnostics and Control of Apicomplexan Parasites
顶复门寄生虫的分子系统发育、诊断和控制
- 批准号:
RGPIN-2016-05826 - 财政年份:2022
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
Molecular Phylogenetics, Diagnostics and Control of Apicomplexan Parasites
顶复门寄生虫的分子系统发育、诊断和控制
- 批准号:
RGPIN-2016-05826 - 财政年份:2021
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
Molecular Phylogenetics, Diagnostics and Control of Apicomplexan Parasites
顶复门寄生虫的分子系统发育、诊断和控制
- 批准号:
RGPIN-2016-05826 - 财政年份:2019
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
Molecular Phylogenetics, Diagnostics and Control of Apicomplexan Parasites
顶复门寄生虫的分子系统发育、诊断和控制
- 批准号:
RGPIN-2016-05826 - 财政年份:2017
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
Molecular Phylogenetics, Diagnostics and Control of Apicomplexan Parasites
顶复门寄生虫的分子系统发育、诊断和控制
- 批准号:
RGPIN-2016-05826 - 财政年份:2016
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
Molecular identification and control of apicomplexan parasites
顶复门寄生虫的分子鉴定和控制
- 批准号:
106453-2011 - 财政年份:2015
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
Molecular identification and control of apicomplexan parasites
顶复门寄生虫的分子鉴定和控制
- 批准号:
106453-2011 - 财政年份:2014
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
Molecular identification and control of apicomplexan parasites
顶复门寄生虫的分子鉴定和控制
- 批准号:
106453-2011 - 财政年份:2013
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
Development of a molecular assay for determining the number and viability of live vaccine organisms
开发确定活疫苗生物体数量和活力的分子测定方法
- 批准号:
461263-2013 - 财政年份:2013
- 资助金额:
$ 2.4万 - 项目类别:
Engage Plus Grants Program
Molecular identification and control of apicomplexan parasites
顶复门寄生虫的分子鉴定和控制
- 批准号:
106453-2011 - 财政年份:2012
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
相似海外基金
Postdoctoral Fellowship: EAR-PF: Novel approaches to evaluating the quality of the global fossil record: the frontier between taphonomy and phylogenetics
博士后奖学金:EAR-PF:评估全球化石记录质量的新方法:埋藏学和系统发育学之间的前沿
- 批准号:
2305564 - 财政年份:2023
- 资助金额:
$ 2.4万 - 项目类别:
Fellowship Award
Mathematical Methods in Linguistics and Phylogenetics
语言学和系统发育学中的数学方法
- 批准号:
RGPIN-2019-06911 - 财政年份:2022
- 资助金额:
$ 2.4万 - 项目类别:
Discovery Grants Program - Individual
An introductory molecular phylogenetics course: integrating molecules with taxonomy and systematics
分子系统发育入门课程:将分子与分类学和系统学相结合
- 批准号:
NE/X003469/1 - 财政年份:2022
- 资助金额:
$ 2.4万 - 项目类别:
Training Grant
Resolving the phylogenetics of the sedge genus Schoenoplectiella
解决莎草属 Schoenoplectiella 的系统发育学问题
- 批准号:
576116-2022 - 财政年份:2022
- 资助金额:
$ 2.4万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Master's
Miami Dade County ASsessment of Phylogenetics to Improve Resource Equity: MD ASPIRE
迈阿密戴德县系统发生学评估以改善资源公平:MD ASPIRE
- 批准号:
10653972 - 财政年份:2022
- 资助金额:
$ 2.4万 - 项目类别:
Real-time phylogenetics using sequential Monte Carlo with tree sequences
使用顺序蒙特卡罗和树序列进行实时系统发育
- 批准号:
EP/W006790/1 - 财政年份:2022
- 资助金额:
$ 2.4万 - 项目类别:
Research Grant
DMS/NIGMS 2: Scalable Bayesian Inference with Applications to Phylogenetics
DMS/NIGMS 2:可扩展贝叶斯推理及其在系统发育学中的应用
- 批准号:
2153704 - 财政年份:2022
- 资助金额:
$ 2.4万 - 项目类别:
Continuing Grant
Miami Dade County ASsessment of Phylogenetics to Improve Resource Equity: MD ASPIRE
迈阿密戴德县系统发生学评估以改善资源公平:MD ASPIRE
- 批准号:
10460007 - 财政年份:2022
- 资助金额:
$ 2.4万 - 项目类别:
CAREER: Polyploid phylogenetics and ploidy-aware taxonomy of Cystopteridaceae: Research and education from the field to museum collections
职业:囊蕨科的多倍体系统发育和倍性感知分类学:从实地到博物馆收藏的研究和教育
- 批准号:
2144011 - 财政年份:2022
- 资助金额:
$ 2.4万 - 项目类别:
Continuing Grant